Abstract
After the chromatographic separation of [methyl-14C]gentamicin major (C) components from a large-scale radioactive fermentation (Lee et al., 1974), [methyl-14C]gentamicin minor (polar) components (A, B, B1, X2, and G-418) were isolated from subsequent chromatography of the remaining antibiotic mixture. When l-[methyl-14C]methionine was added at the onset of biosynthesis of the gentamicin components, incorporation of label into the minor components preceded incorporation into the major components. Degradation occurred when [methyl-14C]gentamicin major components (C1, C2 and C1a) were added respectively to the gentamicin-producing culture medium and shaken.
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