Skip to main content
NCBI home page
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1976 Mar;9(3):414–417. doi: 10.1128/aac.9.3.414

Use of Sodium Polyanethol Sulfonate to Selectively Inhibit Aminoglycoside and Polymyxin Antibiotics in a Rapid Blood Level Antibiotic Assay

Stephen C Edberg 1, Cecil J Bottenbley 1, Kalman Gam 1
PMCID: PMC429545  PMID: 176933

Abstract

Sodium polyanethol sulfonate inhibits aminoglycoside and polymyxin classes of antibiotics in direct proportion to its concentration. Aminoglycoside and polymyxin class antibiotics are selectively inactivated; penicillin, including the semisynthetic penicillins, cephalothin, chloramphenicol, clindamycin, tetracycline, erythromycin, and vancomycin are not inhibited. By incorporating sodium polyanethol sulfonate directly into the test medium it is possible, in a 4-h antibiotic blood level assay, to selectively obviate the activity of the aminoglycosides and polymyxins to determine the concentration of other antibiotics present in the same serum sample.

Full text

PDF
414

Images in this article

416Image
on p.416

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Belding M. E., Klebanoff S. J. Effect of sodium polyanetholesulfonate on antimicrobial systems in blood. Appl Microbiol. 1972 Nov;24(5):691–698. doi: 10.1128/am.24.5.691-698.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Edberg S. C., Chu A. Determining antibiotic levels in the blood. Am J Med Technol. 1975 Mar;41(3):99–105. [PubMed] [Google Scholar]
  3. Eng J., Iveland H. Inhibitory effect in vitro of sodium polyanethol sulfonate on the growth of Neisseria meningitidis. J Clin Microbiol. 1975 May;1(5):444–447. doi: 10.1128/jcm.1.5.444-447.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ervin F. R., Bullock W. E., Jr Simple assay for clindamycin in the presence of aminoglycosides. Antimicrob Agents Chemother. 1974 Dec;6(6):831–835. doi: 10.1128/aac.6.6.831. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lund M. E., Blazevic D. J., Matsen J. M. Rapid gentamicin bioassay using a multiple-antibiotic-resistant strain of Klebsiella pneumoniae. Antimicrob Agents Chemother. 1973 Nov;4(5):569–573. doi: 10.1128/aac.4.5.569. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. MAY J. R., VOUREKA A. E., FLEMING A. Some problems in the tritration of streptomycin. Br Med J. 1947 May 10;1(4505):627–630. doi: 10.1136/bmj.1.4505.627. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Rosner R. Effect of various anticoagulants and no anticoagulant on ability to isolate bacteria directly from parallel clinical blood specimens. Am J Clin Pathol. 1968 Feb;49(2):216–219. doi: 10.1093/ajcp/49.2.216. [DOI] [PubMed] [Google Scholar]
  8. Sabath L. D., Casey J. I., Ruch P. A., Stumpf L. L., Finland M. Rapid microassay of gentamicin, kanamycin, neomycin, streptomycin, and vancomycin in serum or plasma. J Lab Clin Med. 1971 Sep;78(3):457–463. [PubMed] [Google Scholar]
  9. Sabath L. D., Toftegaard I. Rapid microassays for clindamycin and gentamicin when present together and the effect of pH and of each on the antibacterial activity of the other. Antimicrob Agents Chemother. 1974 Jul;6(1):54–59. doi: 10.1128/aac.6.1.54. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Traub W. H. Antagonism of polymyxin B and kanamycin sulfate by Liquoid (sodium polyanetholsulfonate) in vitro. Experientia. 1969 Feb 15;25(2):206–207. doi: 10.1007/BF01899127. [DOI] [PubMed] [Google Scholar]
  11. Traub W. H., Lowrance B. L. Anticomplementary, anticoagulatory, and serum-protein precipitating activity of sodium polyanetholsulfonate. Appl Microbiol. 1970 Sep;20(3):465–468. doi: 10.1128/am.20.3.465-468.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Traub W. H., Lowrance B. L. Media-dependent antagonism of gentamicin sulfate by Liquoid (sodium polyanetholsulfonate). Experientia. 1969 Nov 15;25(11):1184–1185. doi: 10.1007/BF01900265. [DOI] [PubMed] [Google Scholar]
  13. YOURASSOWSKY E. COURBES DE CROISSANCEM V/ COURBES DE CROISSANCE MICROBIENNE DE STAPHYLOCOCCUS AUREUS, KLEBSIELLA PNEUMONIAE, ESCHERICHIA COLI, STREPTOCOCCUS FAECALIS SUR LE PLASMA DE COBAYE. C R Seances Soc Biol Fil. 1964;158:215–218. [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES