Skip to main content
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1976 Mar;9(3):540–543. doi: 10.1128/aac.9.3.540

In Vitro Susceptibility of Varicella Zoster Virus to Adenine Arabinoside and Hypoxanthine Arabinoside

Yvonne J Bryson 1, James D Connor 1
PMCID: PMC429566  PMID: 176937

Abstract

The in vitro susceptibility of varicella zoster (VZ) isolates to adenine arabinoside (ara-A) and hypoxanthine arabinoside (ara-Hx) was determined in human skin fibroblasts. Using a system in which deamination was inhibited, and using a modified plaque reduction method, the antiviral activities of ara-A and ara-Hx were separated. The plaque inhibitory concentration of ara-A for all VZ isolates tested was 1 to 2 μg/ml and 80 to 100 μg/ml for ara-Hx. A comparison of antiviral activity based upon total plaque suppression gave a rate of 40 to 50:1, in favor of ara-A.

Full text

PDF
540

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Connor J. D., Sweetman L., Carey S., Stuckey M. A., Buchanan R. Effect of adenosine deaminase upon the antiviral activity in vitro of adenine arabinoside for vaccinia virus. Antimicrob Agents Chemother. 1974 Nov;6(5):630–636. doi: 10.1128/aac.6.5.630. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Fiala M., Chow A. W., Miyasaki K., Guze L. B. Susceptibility of herpesviruses to three nucleoside analogues and their combinations and enhancement of the antiviral effect of acid pH. J Infect Dis. 1974 Jan;129(1):82–85. doi: 10.1093/infdis/129.1.82. [DOI] [PubMed] [Google Scholar]
  3. Gershon A., Cosio L., Brunell P. A. Observations on the growth of varicella-zoster virus in human diploid cells. J Gen Virol. 1973 Jan;18(1):21–31. doi: 10.1099/0022-1317-18-1-21. [DOI] [PubMed] [Google Scholar]
  4. Johnson M. T., Buchanan R. A., Luby J. P., Mikulec D. Treatment of varicella-zoster virus infections with adenine arabinoside. J Infect Dis. 1975 Mar;131(3):225–229. doi: 10.1093/infdis/131.3.225. [DOI] [PubMed] [Google Scholar]
  5. MELNICK J. L., MIDULLA M., WIMBERLY I., BARRERA-ORO J. G., LEVY B. M. A NEW MEMBER OF THE HERPESVIRUS GROUP ISOLATED FROM SOUTH AMERICAN MARMOSETS. J Immunol. 1964 Apr;92:596–601. [PubMed] [Google Scholar]
  6. RAPP F., BENYESH-MELNICK M. Plaque assay for measurement of cells infected with zoster virus. Science. 1963 Aug 2;141(3579):433–434. doi: 10.1126/science.141.3579.433. [DOI] [PubMed] [Google Scholar]
  7. RAWLS W. E., COHEN R. A., HERRMANN E. C., Jr INHIBITION OF VARICELLA VIRUS BY 5-IODO-2'-DEOXYURIDINE. Proc Soc Exp Biol Med. 1964 Jan;115:123–127. doi: 10.3181/00379727-115-28848. [DOI] [PubMed] [Google Scholar]
  8. Schabel F. M., Jr The antiviral activity of 9-beta-D-arabinofuranosyladenine (ARA-A). Chemotherapy. 1968;13(6):321–338. doi: 10.1159/000220567. [DOI] [PubMed] [Google Scholar]
  9. WELLER T. H. Serial propagation in vitro of agents producing inclusion bodies derived from varicella and herpes zoster. Proc Soc Exp Biol Med. 1953 Jun;83(2):340–346. doi: 10.3181/00379727-83-20354. [DOI] [PubMed] [Google Scholar]
  10. WELLER T. H., WITTON H. M., BELL E. J. The etiologic agents of varicella and herpes zoster; isolation, propagation, and cultural characteristics in vitro. J Exp Med. 1958 Dec 1;108(6):843–868. doi: 10.1084/jem.108.6.843. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES