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. 1976 Apr;9(4):639–645. doi: 10.1128/aac.9.4.639

Sensitization of Colicin K-Treated Bacteria By Sodium Dodecyl Sulfate: Presence of Free Colicin in Colicin K-Treated Cultures of Escherichia coli

Danièle Cavard a,1
PMCID: PMC429590  PMID: 773302

Abstract

A method of assaying colicin K is described. It makes use of two properties of sodium dodecyl sulfate to protect bacteria against colicin action and to dissolve those bacteria on which colicin K had started its action. By this method, the kinetics of bacterial killing by colicin K have been measured directly in the treated culture without intervening dilution. The kinetics are exponential with time and are a function of the colicin multiplicity, as described previously, but do not reach a final plateau. At any time during colicin treatment, free colicin is found in the medium. Procedures that eliminate or destroy this free colicin, such as centrifugation and resuspension of the treated bacteria in a fresh medium, or addition of trypsin or sodium dodecyl sulfate to the treated culture stop bacterial killing.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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