Skip to main content
NCBI home page
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1976 May;9(5):754–760. doi: 10.1128/aac.9.5.754

Quantitation of Antibiotics Using High-Pressure Liquid Chromatography: Tetracycline

Ingrid Nilsson-Ehle †,‡, Thomas T Yoshikawa †,‡, Michael C Schotz *, Lucien B Guze †,‡
PMCID: PMC429616  PMID: 821388

Abstract

A method for quantitative determination of tetracycline in serum, using high-pressure liquid chromatography, is reported. After extraction of the drug from serum, using methanol-trichloroacetic acid solution, tetracycline was separated by reverse-phase chromatography. Quantitation of tetracycline was based on a linear relationship between peak heights in the chromatograms and known concentrations of the drug in the original serum samples. Serum tetracycline concentrations as low as 0.3 μg/ml could be accurately measured. Serum samples obtained after a single intravenous injection of tetracycline to three human volunteers and six dogs were assayed by microbiological and chemical assays. Correlation coefficients of 0.95 and 0.97, respectively, were found. This chemical method is rapid (less than 30 min), accurate, sensitive, and reproducible, and it seems feasible for routine clinical use.

Full text

PDF
754

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blair A. D., Forrey A. W., Meijsen B. T., Cutler R. E. Assay of flucytosine and furosemide by high-pressure liquid chromatography. J Pharm Sci. 1975 Aug;64(8):1334–1339. doi: 10.1002/jps.2600640816. [DOI] [PubMed] [Google Scholar]
  2. Buhs R. P., Maxim T. E., Allen N., Jacob T. A., Wolf F. J. Analysis of cefoxitin, cephalothin and their deacylated metabolites in human urine by high-performance liquid chromatography. J Chromatogr. 1974 Nov 6;99(0):609–618. doi: 10.1016/s0021-9673(00)90889-6. [DOI] [PubMed] [Google Scholar]
  3. Cooper M. J., Anders M. W., Mirkin B. L. Ion-pair extraction and high-speed liquid chromatography of cephalothin and deacetylcephalothin in human serum and urine. Drug Metab Dispos. 1973 Sep-Oct;1(5):659–662. [PubMed] [Google Scholar]
  4. Edberg S. C., Chu A. Determining antibiotic levels in the blood. Am J Med Technol. 1975 Mar;41(3):99–105. [PubMed] [Google Scholar]
  5. Haas M. J., Davies J. Enzymatic acetylation as a means of determining serum aminoglycoside concentrations. Antimicrob Agents Chemother. 1973 Oct;4(4):497–499. doi: 10.1128/aac.4.4.497. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Inaba T., Besley M. E., Chow E. J. Determination of sulfinpyrazone in serum by high-performance liquid chromatography. J Chromatogr. 1975 Jan 29;104(1):165–169. doi: 10.1016/s0021-9673(01)85501-1. [DOI] [PubMed] [Google Scholar]
  7. KUNIN C. M., FINLAND M. Clinical pharmacology of the tetracycline antibiotics. Clin Pharmacol Ther. 1961 Jan-Feb;2:51–69. doi: 10.1002/cpt19612151. [DOI] [PubMed] [Google Scholar]
  8. Kelly R. G., Hoyt K. D. A method of analysis for the individual tetracyclines of a mixture in biological fluids. Z Klin Chem Klin Biochem. 1969 Mar;7(2):152–154. doi: 10.1515/cclm.1969.7.2.152. [DOI] [PubMed] [Google Scholar]
  9. Kunin C. M. A guide to use of antibiotics in patients with renal disease. A table of recommended doses and factors governing serum levels. Ann Intern Med. 1967 Jul;67(1):151–158. doi: 10.7326/0003-4819-67-1-151. [DOI] [PubMed] [Google Scholar]
  10. Lever M. Improved fluorometric determination of tetracyclines. Biochem Med. 1972 Jun;6(3):216–222. doi: 10.1016/0006-2944(72)90041-5. [DOI] [PubMed] [Google Scholar]
  11. Mahon W. A., Ezer J., Wilson T. W. Radioimmunoassay for measurement of gentamicin in blood. Antimicrob Agents Chemother. 1973 May;3(5):585–589. doi: 10.1128/aac.3.5.585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Powis G. A study of the interaction of tetracycline with human serum lipoproteins and albumin. J Pharm Pharmacol. 1974 Feb;26(2):113–118. doi: 10.1111/j.2042-7158.1974.tb09235.x. [DOI] [PubMed] [Google Scholar]
  13. Stevens P., Young L. S., Hewitt W. L. Improved acetylating radioenzymatic assay of amikacin, tobramycin, and sisomicin in serum. Antimicrob Agents Chemother. 1975 Mar;7(3):374–376. doi: 10.1128/aac.7.3.374. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Thompson R. D., Nagasawa H. T., Jenne J. W. Determination of theophylline and its metabolites in human urine and serum by high-pressure liquid chromatography. J Lab Clin Med. 1974 Oct;84(4):584–593. [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES