Skip to main content
NCBI home page
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1976 Jul;10(1):75–79. doi: 10.1128/aac.10.1.75

Susceptibility of Prototheca Species to Antifungal Agents

Ester Segal 1, Arvind A Padhye 1, Libero Ajello 1
PMCID: PMC429691  PMID: 984758

Abstract

Twenty isolates of Prototheca filamenta, Prototheca moriformis, Prototheca stagnora, Prototheca wickerhamii, and Prototheca zopfii were tested for in vitro susceptibility to five commonly used antifungal agents: amphotericin B, 5-fluorocytosine, griseofulvin, miconazole, and nystatin. The results revealed resistance to griseofulvin of all the Prototheca isolates tested and an inhibitory effect on P. filamenta by high 5-fluorocytosine concentrations (minimal inhibitory concentration [MIC] = 12.5 to 100 μg/ml; minimal fungicidal or algacidal concentration [MFC/MAC] = 50 to 100 μg/ml). P. filamenta isolates were also susceptible to miconazole (MIC = 0.1 to 0.5 μg/ml, MFC/MAC = 0.5 to 1 μg/ml); isolates of the other Prototheca species varied in regard to miconazole activity from susceptible to resistant (MIC = 1 − >100 μg/ml, MFC/MAC = 5 − >100 μg/ml). The Prototheca isolates revealed an in vitro susceptibility to the polyene antifungal agents, amphotericin B, and nystatin (MIC = 0.09 to 3.12 μg/ml and 0.19 to 12.5 μg/ml, respectively; MFC/MAC = 0.19 to 25 μg/ml and 0.75 to 25 μg/ml, respectively).

Full text

PDF
75

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Carlton W. W., Austin L. Ocular protothecosis in a dog. Vet Pathol. 1973;10(3):274–280. doi: 10.1177/030098587301000310. [DOI] [PubMed] [Google Scholar]
  2. Cox G. E., Wilson J. D., Brown P. Protothecosis: a case of disseminated algal infection. Lancet. 1974 Aug 17;2(7877):379–382. doi: 10.1016/s0140-6736(74)91760-7. [DOI] [PubMed] [Google Scholar]
  3. Davies R. R., Wilkinson J. L. Human protothecosis: supplementary studies. Ann Trop Med Parasitol. 1967 Mar;61(1):112–114. doi: 10.1080/00034983.1967.11686466. [DOI] [PubMed] [Google Scholar]
  4. Gentles J. C., Jones G. R., Roberts D. T. Efficacy of miconazole in the topical treatment of tinea pedis in sportsmen. Br J Dermatol. 1975 Jul;93(1):79–84. doi: 10.1111/j.1365-2133.1975.tb06480.x. [DOI] [PubMed] [Google Scholar]
  5. Holt R. J. Recent developments in antimycotic chemotherapy. Infection. 1974;2(2):95–107. doi: 10.1007/BF01642028. [DOI] [PubMed] [Google Scholar]
  6. Klintworth G. K., Fetter B. F., Nielsen H. S., Jr Protothecosis, an algal infection: report of a case in man. J Med Microbiol. 1968 Nov;1(2):211–216. doi: 10.1099/00222615-1-2-211. [DOI] [PubMed] [Google Scholar]
  7. Lee W. S., Lagios M. D., Leonards R. Wound infection by Prototheca wickerhamii, a saprophytic alga pathogenic for man. J Clin Microbiol. 1975 Jul;2(1):62–66. doi: 10.1128/jcm.2.1.62-66.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Levine H. B., Stevens D. A., Cobb J. M., Gebhardt A. E. Miconazole in coccidioidomycosis. I. Assays of activity in mice and in vitro. J Infect Dis. 1975 Oct;132(4):407–414. doi: 10.1093/infdis/132.4.407. [DOI] [PubMed] [Google Scholar]
  9. Proost J. M., Maes-Dockx F. M., Nelis M. O., Van Cutsem J. M. Miconazole in the treatment of mycotic vulvovaginitis. Am J Obstet Gynecol. 1972 Mar;112(5):688–692. doi: 10.1016/0002-9378(72)90796-x. [DOI] [PubMed] [Google Scholar]
  10. Segal E., Romano A., Eylan E., Stein R. Experimental and clinical studies of 5-fluorocytosine activity in Candida ocular infections. I. In vitro activity of 5-fluorocytosine on Candida species isolated from ocular infections. Chemotherapy. 1975;21(6):358–366. doi: 10.1159/000221880. [DOI] [PubMed] [Google Scholar]
  11. Shadomy S. In vitro studies with 5-fluorocytosine. Appl Microbiol. 1969 Jun;17(6):871–877. doi: 10.1128/am.17.6.871-877.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sudman M. S., Kaplan W. Identification of the Prototheca species by immunofluorescence. Appl Microbiol. 1973 Jun;25(6):981–990. doi: 10.1128/am.25.6.981-990.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sudman M. S. Protothecosis. A critical review. Am J Clin Pathol. 1974 Jan;61(1):10–19. doi: 10.1093/ajcp/61.1.10. [DOI] [PubMed] [Google Scholar]
  14. Van Kruiningen H. J. Protothecal enterocolitis in a dog. J Am Vet Med Assoc. 1970 Jul 1;157(1):56–63. [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES