Skip to main content
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1976 Jul;10(1):182–184. doi: 10.1128/aac.10.1.182

Nalidixic Acid Inhibition of Post-Ultraviolet Recovery in Escherichia coli K-12: Requirement for recBC Function

Michael A Purdy 1, K Lemone Yielding 1
PMCID: PMC429711  PMID: 791087

Abstract

Various repair-deficient mutants of Escherichia coli K-12 were tested for the impairment of post-ultraviolet survival by nalidixic acid. These studies have shown that the target of this nalidixic acid effect is dictated by the recB and recC genes and may be due to the binding of nalidixic acid to exonuclease V. Furthermore, this effect cannot be correlated with either recombination proficiency or excision repair alone.

Full text

PDF
182

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bourguignon G. J., Levitt M., Sternglanz R. Studies on the mechanism of action of nalidixic acid. Antimicrob Agents Chemother. 1973 Oct;4(4):479–486. doi: 10.1128/aac.4.4.479. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boyle J. V., Cook T. M., Goss W. A. Mechanism of action of nalidixic acid on Escherichia coli. Vi. Cell-free studies. J Bacteriol. 1969 Jan;97(1):230–236. doi: 10.1128/jb.97.1.230-236.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Eberle H., Masker W. Effect of nalidixic acid on semionservative replication and repair synthesis after ultraviolet irradiation in Escherichia coli. J Bacteriol. 1971 Mar;105(3):908–912. doi: 10.1128/jb.105.3.908-912.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gefter M. L., Hirota Y., Kornberg T., Wechsler J. A., Barnoux C. Analysis of DNA polymerases II and 3 in mutants of Escherichia coli thermosensitive for DNA synthesis. Proc Natl Acad Sci U S A. 1971 Dec;68(12):3150–3153. doi: 10.1073/pnas.68.12.3150. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Goldmark P. J., Linn S. Purification and properties of the recBC DNase of Escherichia coli K-12. J Biol Chem. 1972 Mar 25;247(6):1849–1860. [PubMed] [Google Scholar]
  6. Howard-Flanders P., Boyce R. P., Theriot L. Three loci in Escherichia coli K-12 that control the excision of pyrimidine dimers and certain other mutagen products from DNA. Genetics. 1966 Jun;53(6):1119–1136. doi: 10.1093/genetics/53.6.1119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Nishida M., Mishima Y., Kawada J., Yielding K. L. Enhancement by nalidixic acid of the thermal susceptibility of the Ts-7 mutant of Escherichia coli TAU-bar. Antimicrob Agents Chemother. 1975 Sep;8(3):384–386. doi: 10.1128/aac.8.3.384. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Nishida M., Nakamura N., Yielding K. L. Inhibition by nalidixic acid of post-uv survival of Escherichia coli. Proc Soc Exp Biol Med. 1976 Feb;151(2):271–274. doi: 10.3181/00379727-151-39190. [DOI] [PubMed] [Google Scholar]
  9. Pedrini A. M., Geroldi D., Siccardi A., Falaschi A. Studies on the mode of action of nalidixic acid. Eur J Biochem. 1972 Feb 15;25(2):359–365. doi: 10.1111/j.1432-1033.1972.tb01704.x. [DOI] [PubMed] [Google Scholar]
  10. Simon T. J., Masker W. E., Hanawalt P. C. Selective inhibition of semiconservative DNA synthesis by nalidixic acid in permeabilized bacteria. Biochim Biophys Acta. 1974 May 17;349(2):271–274. doi: 10.1016/0005-2787(74)90089-6. [DOI] [PubMed] [Google Scholar]
  11. Willetts N. S., Clark A. J., Low B. Genetic location of certain mutations conferring recombination deficiency in Escherichia coli. J Bacteriol. 1969 Jan;97(1):244–249. doi: 10.1128/jb.97.1.244-249.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Wright M., Buttin G., Hurwitz J. The isolation and characterization from Escherichia coli of an adenosine triphosphate-dependent deoxyribonuclease directed by rec B, C genes. J Biol Chem. 1971 Nov;246(21):6543–6555. [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES