Skip to main content
NCBI home page
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1976 Jul;10(1):185–187. doi: 10.1128/aac.10.1.185

Inhibition of L5178Y Cells in Culture by Methotrexate and Antibiotics

P L Chello 1,2,1, H W Bruckner 1,2,2
PMCID: PMC429712  PMID: 984751

Abstract

Methotrexate, tetracycline, gentamicin, streptomycin, and penicillin inhibited the growth of L5178Y murine leukemia cells in culture with I50 (concentration of drug that caused a 50% inhibition of growth at 72 h) values of 0.0028 μg/ml (6.2 × 10−9 M), 7.9 μg/ml, 200 μg/ml, 1,700 μg/ml, and 3,000 μg/ml (5,000 U/ml), respectively. At concentrations achieved clinically or utilized in the laboratory, the antibiotics did not alter the I50 of methotrexate.

Full text

PDF
185

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chello P. L., Bertino J. R. Dependence of 5-methyltetrahydrofolate utilization by L5178Y murine leukemia cells in vitro on the presence of hydroxycobalamin and transcobalamin II. Cancer Res. 1973 Aug;33(8):1898–1904. [PubMed] [Google Scholar]
  2. Chello P. L., Bertino J. R. Effect of methionine deprivation on L5178Y murine leukemia cells in culture interference with the antineoplastic effect of methotrexate. Biochem Pharmacol. 1976 Apr 15;25(8):889–892. doi: 10.1016/0006-2952(76)90309-9. [DOI] [PubMed] [Google Scholar]
  3. FISCHER G. A. Increased levels of folic acid reductase as a mechanism of resistance to amethopterin in leukemic cells. Biochem Pharmacol. 1961 Jul;7:75–77. doi: 10.1016/0006-2952(61)90128-9. [DOI] [PubMed] [Google Scholar]
  4. FISCHER G. A., SARTORELLI A. C. DEVELOPMENT, MAINTENANCE AND ASSAY OF DRUG RESISTANCE. Methods Med Res. 1964;10:247–262. [PubMed] [Google Scholar]
  5. Rodriguez V., Bodey G. P., Valdivieso M., Feld R. Clinical pharmacology of sisomicin. Antimicrob Agents Chemother. 1975 Jan;7(1):38–41. doi: 10.1128/aac.7.1.38. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Weisblum B., Davies J. Antibiotic inhibitors of the bacterial ribosome. Bacteriol Rev. 1968 Dec;32(4 Pt 2):493–528. [PMC free article] [PubMed] [Google Scholar]
  7. Zager R. F., Frisby S. A., Oliverio V. T. The effects of antibiotics and cancer chemotherapeutic agents on the cellular transport and antitumor activity of methotrexate in L1210 murine leukemia. Cancer Res. 1973 Jul;33(7):1670–1676. [PubMed] [Google Scholar]
  8. Zaharko D. S., Bruckner H., Oliverio V. T. Antibiotics alter methotrexate metabolism and excretion. Science. 1969 Nov 14;166(3907):887–888. doi: 10.1126/science.166.3907.887. [DOI] [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES