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. Author manuscript; available in PMC: 2015 Jan 16.
Published in final edited form as: Nature. 2009 Nov 25;462(7276):1079–1082. doi: 10.1038/nature08620

Figure 2.

Figure 2

Designed FeBMb has NOR activity in the presence of Fe2+ and NO. Control proteins show no evidence of NO activity under identical conditions. A) UV-vis spectra of 10 μM of deoxy FeBMb (a), deoxy wtMb (b), and deoxy FeBMb(-Glu) (c) in the absence (black spectra) or presence (red spectra) of 2 eq Fe2+. B) UV-vis spectra of the NO reaction of 10 μM of deoxy FeBMb (a), deoxy wtMb (b), and deoxy FeBMb(-Glu) (c) in the absence (black spectra) or presence (red spectra) of 2 eq Fe2+ after 20 min of incubation with ~17 eq NO. The deoxy protein was formed by reaction of met protein with excess dithionite in a glove box. Gel filtration purification was used to remove excess dithionite to preclude its reaction with NO.