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. 1976 Aug;10(2):258–264. doi: 10.1128/aac.10.2.258

New Plasmid-Mediated Nucleotidylation of Aminoglycoside Antibiotics in Staphylococcus aureus

F Le Goffic *, A Martel †, M L Capmau †, B Baca †, P Goebel †, H Chardon †, C J Soussy †, J Duval †, D H Bouanchaud †
PMCID: PMC429732  PMID: 984767

Abstract

A wild-type strain of Staphylococcus aureus, which inactivates a wide variety of aminoglycosides (except the gentamicin components), has been found to harbor a plasmid (RAp01) that mediates the biosynthesis of a nucleotidyltransferase. This enzyme modifies the 4′-hydroxy function of these antibiotics. The plasmid has been studied, the enzyme responsible for this resistance pattern has been isolated by affinity chromatography, and its kinetics and physicochemistry have been characterized. The target of this enzyme has also been located by demonstrating the structure of one inactivated compound, 4′-(O)-adenylyltobramycin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benveniste R., Davies J. Mechanisms of antibiotic resistance in bacteria. Annu Rev Biochem. 1973;42:471–506. doi: 10.1146/annurev.bi.42.070173.002351. [DOI] [PubMed] [Google Scholar]
  2. Bouanchaud D. H., Scavizzi M. R., Chabbert Y. A. Elimination by ethidium bromide of antibiotic resistance in enterobacteria and staphylococci. J Gen Microbiol. 1968 Dec;54(3):417–425. doi: 10.1099/00221287-54-3-417. [DOI] [PubMed] [Google Scholar]
  3. Davies J. E., Rownd R. Transmissible multiple drug resistance in Enterobacteriaceae. Science. 1972 May 19;176(4036):758–768. doi: 10.1126/science.176.4036.758. [DOI] [PubMed] [Google Scholar]
  4. Doi O., Miyamoto M., Tanaka N., Umezawa H. Inactivation and phosphorylation of kanamycin by drug-resistant Staphylococcus aureus. Appl Microbiol. 1968 Sep;16(9):1282–1284. doi: 10.1128/am.16.9.1282-1284.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ingram L. C., Richmond M. H., Sykes R. B. Molecular characterization of the R factors implicated in the carbenicillin resistance of a sequence of Pseudomonas aeruginosa strains isolated from burns. Antimicrob Agents Chemother. 1973 Feb;3(2):279–288. doi: 10.1128/aac.3.2.279. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kobayashi F., Koshi T., Eda J., Yoshimura Y., Mitsuhashi S. Lividomycin resistance in staphylococci by enzymatic phosphorylation. Antimicrob Agents Chemother. 1973 Jul;4(1):1–5. doi: 10.1128/aac.4.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kondo S., Yamamoto H., Naganawa H., Umezawa H. Isolation and characterization of lividomycin A inactivated by Pseudomonas aeruginosa and Escherichia coli carrying R factor. J Antibiot (Tokyo) 1972 Aug;25(8):483–484. doi: 10.7164/antibiotics.25.483. [DOI] [PubMed] [Google Scholar]
  8. Le Goffic F., Martel A., Witchitz J. 3-N enzymatic acetylation of gentamicin, tobramycin, and kanamycin by Escherichia coli carrying an R factor. Antimicrob Agents Chemother. 1974 Dec;6(6):680–684. doi: 10.1128/aac.6.6.680. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Le Goffic F., Moreau N., Chevereau M. Purification par chromatographie d'affinité d'une acétyltransférase et d'une phosphotransférase inactivant les antibiotiques aminosidiques. Biochimie. 1973;55(10):1183–1186. [PubMed] [Google Scholar]
  10. Le Goffic F., Moreau N. Purification by affinity chromatography of an enzyme involved in gentamicin inactivation. FEBS Lett. 1973 Feb 1;29(3):289–291. doi: 10.1016/0014-5793(73)80041-9. [DOI] [PubMed] [Google Scholar]
  11. Novick R. P., Bouanchaud D. The problems of drug-resistant pathogenic bacteria. Extrachromosomal nature of drug resistance in Staphylococcus aureus. Ann N Y Acad Sci. 1971 Jun 11;182:279–294. doi: 10.1111/j.1749-6632.1971.tb30664.x. [DOI] [PubMed] [Google Scholar]
  12. Soussy C. J., Bouanchaud D. H., Fouace J., Dublanchet A., Duval J. A gentamycin resistance plasmid in Staphylococcus aureus. Ann Microbiol (Paris) 1975 Jul-Aug;126B(1):91–94. [PubMed] [Google Scholar]
  13. Stiffler P. W., Sweeney H. M., Schneider M., Cohen S. Isolation and characterization of a kanamycin resistance plasmid from Staphylococcus aureus. Antimicrob Agents Chemother. 1974 Oct;6(4):516–520. doi: 10.1128/aac.6.4.516. [DOI] [PMC free article] [PubMed] [Google Scholar]

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