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. 1976 Aug;10(2):347–353. doi: 10.1128/aac.10.2.347

Chloramphenicol: an Enzymological Microassay

Paul S Lietman *, Thomas J White †,1, William V Shaw †,2
PMCID: PMC429746  PMID: 791098

Abstract

A new assay for chloramphenicol in biological fluids has been developed that offers sensitivity, specificity, precision, accuracy, economy, and ease of performance. The assay is based on the enzymological acetylation of chloramphenicol catalyzed by an R factor-mediated enzyme. [14C]acetyl coenzyme A serves as the donor of the labeled acetyl group, and the product, [14C]acetoxychloramphenicol, is separated from the labeled precursor by utilizing its preferential extraction into benzene. The product is then quantified by liquid scintillation counting. This assay measures chloramphenicol concentrations in both plasma and other biological specimens and in the presence of other antibiotics, hemolysis, or jaundice. Its rapidity and ease of performance are useful for clinical laboratories, and its sensitivity allows determinations on 10 μl of plasma.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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