Extended Data Figure 6.

pHAM2::YFP-HAM2 (pHAM2::YPET-HAM2)complemented the ham1,2,4 mutant and was expressed in the center of SAMs. The early termination phenotype ofham1;2;4(a, b) was completely complemented by YPET-HAM2 driven by the HAM2 promoter and 3′UTR (c), indicating the promoter used for HAM2 transcriptional and translational reporters are functional and the fusion protein (YPET-HAM2) is also functional in vivo. Arrows in (a, b) indicate early-terminated apices. Bars = 10 mm (a–c). (d–e) Different Z sections from the same SAM in ham1,2,4 [pHAM2::YPET-HAM2] plant for Fig. 3 (m–n) show expression of pHAM2::YPET-HAM2 translationalmarker (green) in L2 (d) and L3 (e), together with PI as counter stain (Red). Bars = 20 μm (d–e). (f) Immunoblot with anti-GFP antibody validates the presence of YFP-HAM2 (YPET-HAM2) in both nuclear lysate and nuclear proteins immunoprecipitated with GFP-Trap from ham1,2,4 [pHAM2::YFP-HAM2] line used in ChIP experiment (Fig. 2, n and o).