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. Author manuscript; available in PMC: 2015 Jul 15.
Published in final edited form as: Nature. 2014 Oct 29;517(7534):360–364. doi: 10.1038/nature13864

Figure 1. Vector design and experimental scheme.

Figure 1

a. The rAAV8 vector encodes a codon-optimized hF9 cDNA and preceding 2A-peptide coding sequence flanked by homology arms spanning the Alb stop codon. Length of the 5′ and 3′ arms are 1.3 and 1.4 Kb, respectively. Following integration by homologous recombination, Alb and hF9 are fused at the DNA and RNA levels, but two separate proteins are produced as the result of ribosomal skipping. b. With respect to the Alb homology arms, the AAV inverse control has hF9 inverted along with the 2A-peptide coding sequence, the adjacent Alb exon and the preceding splice junction. Thin white lines: Alb introns; dark gray boxes: Alb exons; white boxes: P2A; white arrows: hF9 transgene; light gray boxes: extragenic DNA; P: proline.