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. 1976 Oct;10(4):613–617. doi: 10.1128/aac.10.4.613

Growth of Physarum gyrosum on Agar Plates and in Liquid Culture1

Richard L Taylor a, M F Mallette a
PMCID: PMC429802  PMID: 10830

Abstract

The physical and nutritional requirements of the antibiotic-producing slime mold Physarum gyrosum were examined to develop a liquid medium for this myxomycete. Liquid culture is desired to expedite a useful scale of production of antibiotic materials for ease of isolation and structure study. Culture conditions were selected to favor antibiotic production rather than maximum growth. The medium devised consisted of 0.010 M potassium phosphate buffer (pH 6.0), 2% bakers' yeast, and 0.2% glucose and was supplemented with either 10−7 M hemoglobin (preferred) or 2.0 ml of live Escherichia coli per 100 ml of culture medium grown to a steady-state population in nutrient broth. The slime mold, which contained some E. coli carried along with the inoculum, was allowed to grow as a surface plasmodium at 20°C in the dark with weekly subculturing for stocks or for 10 days for antibiotic production. P. gyrosum produced the same antibiotic materials when grown in liquid medium as it did when grown on agar plates. A seeded plate disk assay against Bacillus cereus was employed to follow antibiotic activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BREWER E. N., KURAISHI S., GARVER J. C., STRONG F. M. MASS CULTURE OF A SLIME MOLD, PHYSARUM POLYCEPHALUM. Appl Microbiol. 1964 Mar;12:161–164. doi: 10.1128/am.12.2.161-164.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CONSIDINE J. M., MALLETTE M. F. PRODUCTION AND PARTIAL PURIFICATION OF ANTIBIOTIC MATERIALS FORMED BY PHYSARUM GYROSUM. Appl Microbiol. 1965 May;13:464–468. doi: 10.1128/am.13.3.464-468.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DANIEL J. W., BABCOCK K. L., SIEVERT A. H., RUSCH H. P. ORGANIC REQUIREMENTS AND SYNTHETIC MEDIA FOR GROWTH OF THE MYXOMYCETE PHYSARUM POLYCEPHALUM. J Bacteriol. 1963 Aug;86:324–331. doi: 10.1128/jb.86.2.324-331.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DANIEL J. W., KELLEY J., RUSCH H. P. Hematin--requiring plasmodial myxomycete. J Bacteriol. 1962 Nov;84:1104–1110. doi: 10.1128/jb.84.5.1104-1110.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DANIEL J. W., RUSCH H. P. The pure culture of Physarum polycephalum on a partially defined soluble medium. J Gen Microbiol. 1961 May;25:47–59. doi: 10.1099/00221287-25-1-47. [DOI] [PubMed] [Google Scholar]
  6. GUTTES E., GUTTES S., RUSCH H. P. Morphological observations on growth and differentation of Physarum polycephalum grown in pure culture. Dev Biol. 1961 Oct;3:588–614. doi: 10.1016/0012-1606(61)90034-3. [DOI] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. SCHOLES P. M. Some observations on the cultivation, fruiting and germination of Fuligo septica. J Gen Microbiol. 1962 Sep;29:137–148. doi: 10.1099/00221287-29-1-137. [DOI] [PubMed] [Google Scholar]
  9. Schroeder H. R., Fergus C. L., Mallette M. F. Culture of Physarium gyrosum. Mycologia. 1974 Mar-Apr;66(2):349–354. [PubMed] [Google Scholar]
  10. Schroeder H. R., Mallette M. F. Isolation and purification of antibiotic material from Physarum gyrosum. Antimicrob Agents Chemother. 1973 Aug;4(2):160–166. doi: 10.1128/aac.4.2.160. [DOI] [PMC free article] [PubMed] [Google Scholar]

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