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. 1976 Oct;10(4):652–656. doi: 10.1128/aac.10.4.652

Radioimmunoassay of Iodinated Tobramycin

Alan Broughton *, James E Strong †, Larry K Pickering †, Gerald P Bodey †
PMCID: PMC429809  PMID: 988782

Abstract

A radioimmunoassay for tobramycin was developed using antisera from rabbits injected with a tobramycin-bovine serum albumin conjugate. The antibody had a relatively high affinity for 125I-labeled tobramycin (1.6 × 109 liters/mol), thus indicating little interference in immunoreactivity by iodination. The radioimmunoassay was highly specific, and only minor cross-reaction occurred with other aminoglycoside antibiotics. The radioimmunoassay was also more sensitive than previously published tobramycin assays (minimum sensitivity, 280 pg), and there was a significant correlation (P < 0.05) with both the radioenzymatic and microbiological assays.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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