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. 2015 Jan 6;144(2):254–262. doi: 10.1111/imm.12366

Figure 2.

Figure 2

Effect of CD52 monoclonal antibody (mAb) on intestinal permeability, tight junction (TJ) structure and composition, long myosin light chain kinase (MLCK) expression and activity in the proximal colon of interleukin-deficient (IL-10−/−) mice 4 weeks after the final drug administration. Measurements of colonic mannitol flux (a) and electrical resistance (b) in wild-type (WT), IL-10−/− (IL-10-KO) and CD52 mAb treatment (CD52 mAb) mice (n = 6). (c) Representative Western blots of colonic lysates for occludin and zona occludens 1 (ZO-1) in WT mice, IL-10−/− mice or CD52 mAb-treated mice. (d) Densitometric analysis of occludin and ZO-1 (n = 4 to 6). Representative immunofluorescence (green) of occludin (e) and ZO-1 (f) in the proximal colon of mice in three groups (200 × magnification, n = 3 or 4). Nuclei are blue, and arrows show the staining of TJ proteins. (g) Representative transmission electron micrograph of mucosa in three groups (n = 3 or 4), Arrows indicate TJ. (h) Representative Western blots of long MLCK, pMLC, and total MLC (MLC) of isolated colonic epithelia (n = 3). Data in this figure are representative of at least three independent experiments. Data were presented as means ± SEM. *P < 0·05 and **P < 0·01 versus IL-10-KO group.