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. 1976 Dec;10(6):926–928. doi: 10.1128/aac.10.6.926

Medium for Use in Antibiotic Susceptibility Testing of Anaerobic Bacteria

Tracy D Wilkins 1, Sarah Chalgren 1
PMCID: PMC429869  PMID: 1008552

Abstract

A medium is described which was designed for use in testing the minimal inhibitory concentration of antibiotics for anaerobic bacteria by agar dilution. It contains: Trypticase (1%), Gelysate (1%), yeast extract (0.5%), glucose (0.1%), pyruvate (0.1%), arginine (0.1%), NaCl (0.5%), hemin (5 μg/ml), vitamin K1 (0.5 μg/ml), agar (1.5%). The medium does not require the addition of blood to support growth of most clinical isolates of anaerobic bacteria.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DOUGLAS H. C. Glycine fermentation by nongas forming anaerobic Micrococci. J Bacteriol. 1951 Oct;62(4):517–518. doi: 10.1128/jb.62.4.517-518.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Foubert E. L., Douglas H. C. Studies on the Anaerobic Micrococci: I. Taxonomic Considerations. J Bacteriol. 1948 Jul;56(1):25–34. [PMC free article] [PubMed] [Google Scholar]
  3. GIBBONS R. J., MACDONALD J. B. Hemin and vitamin K compounds as required factors for the cultivation of certain strains of Bacteroides melaninogenicus. J Bacteriol. 1960 Aug;80:164–170. doi: 10.1128/jb.80.2.164-170.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. QUINTO G., SEBALD II. IDENTIFICATION OF THREE HEMIN-REQUIRING BACTEROIDES STRAINS. Am J Med Technol. 1964 Nov-Dec;30:381–384. [PubMed] [Google Scholar]
  5. ROGOSA M. THE GENUS VEILLONELLA. I. GENERAL CULTURAL, ECOLOGICAL, AND BIOCHEMICAL CONSIDERATIONS. J Bacteriol. 1964 Jan;87:162–170. doi: 10.1128/jb.87.1.162-170.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. SCHAEDLER R. W., DUBOS R., COSTELLO R. THE DEVELOPMENT OF THE BACTERIAL FLORA IN THE GASTROINTESTINAL TRACT OF MICE. J Exp Med. 1965 Jul 1;122:59–66. doi: 10.1084/jem.122.1.59. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Sperry J. F., Wilkins T. D. Arginine, a growth-limiting factor for Eubacterium lentum. J Bacteriol. 1976 Aug;127(2):780–784. doi: 10.1128/jb.127.2.780-784.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Stargel D., Thompson F. S., Phillips S. E., Lombard G. L., Dowell V. R., Jr Modification of the Minitek Miniaturized Differentiation System for characterization of anaerobic bacteria. J Clin Microbiol. 1976 Mar;3(3):291–301. doi: 10.1128/jcm.3.3.291-301.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Sutter V. L., Kwok Y., Finegold S. M. Susceptibility of Bacteroides fragilis to six antibiotics determined by standardized antimicrobial disc susceptibility testing. Antimicrob Agents Chemother. 1973 Feb;3(2):188–193. doi: 10.1128/aac.3.2.188. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Varel V. H., Bryant M. P. Nutritional features of Bacteroides fragilis subsp. fragilis. Appl Microbiol. 1974 Aug;28(2):251–257. doi: 10.1128/am.28.2.251-257.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]

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