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. 1977 Aug;12(2):219–225. doi: 10.1128/aac.12.2.219

Spread of a Single Plasmid Clone to an Untreated Individual from a Person Receiving Prolonged Tetracycline Therapy

V Petrocheilou 1, M H Richmond 1, P M Bennett 1
PMCID: PMC429888  PMID: 332073

Abstract

The aerobic gram-negative intestinal flora of two individuals living in close proximity was followed for 17 months. One of these persons was receiving a prolonged tetracycline treatment for acne vulgaris and was colonized by tetracycline-resistant Escherichia coli strains throughout the survey. The other person studied received no antibiotics during the period in question, but was frequently found to excrete a number of E. coli strains harboring tetracycline-resistant plasmids. The two E. coli strains (O75.H7 and O23.H16) excreted from both persons most frequently were indistinguishable, and so were the R-plasmids they carried. This suggests that R-plasmid-carrying E. coli may spread from individuals under treatment to close relatives that have not been treated.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Achtman M., Willetts N., Clark A. J. Beginning a genetic analysis of conjugational transfer determined by the F factor in Escherichia coli by isolation and characterization of transfer-deficient mutants. J Bacteriol. 1971 May;106(2):529–538. doi: 10.1128/jb.106.2.529-538.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bennett P. M., Richmond M. H. Translocation of a discrete piece of deoxyribonucleic acid carrying an amp gene between replicons in Eschericha coli. J Bacteriol. 1976 Apr;126(1):1–6. doi: 10.1128/jb.126.1.1-6.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Datta N., Faiers M. C., Reeves D. S., Brumfitt W., Orskov F., Orskov I. R Factors in Escherichia coli in faeces after oral chemotherapy in general practice. Lancet. 1971 Feb 13;1(7694):312–315. doi: 10.1016/s0140-6736(71)91042-7. [DOI] [PubMed] [Google Scholar]
  4. Grinsted J., Saunders J. R., Ingram L. C., Sykes R. B., Richmond M. H. Properties of a R factor which originated in Pseudomonas aeruginosa 1822. J Bacteriol. 1972 May;110(2):529–537. doi: 10.1128/jb.110.2.529-537.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hartley C. L., Howe K., Linton A. H., Linton K. B., Richmond M. H. Distribution of R plasmids among the O-antigen types of Escherichia coli isolated from human and animal sources. Antimicrob Agents Chemother. 1975 Aug;8(2):122–131. doi: 10.1128/aac.8.2.122. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hartley C. L., Richmond M. H. Antibiotic resistance and survival of E coli in the alimentary tract. Br Med J. 1975 Oct 11;4(5988):71–74. doi: 10.1136/bmj.4.5988.71. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Humphreys G. O., Willshaw G. A., Anderson E. S. A simple method for the preparation of large quantities of pure plasmid DNA. Biochim Biophys Acta. 1975 Apr 2;383(4):457–463. doi: 10.1016/0005-2787(75)90318-4. [DOI] [PubMed] [Google Scholar]
  8. Matthew M., Hedges R. W. Analytical isoelectric focusing of R factor-determined beta-lactamases: correlation with plasmid compatibility. J Bacteriol. 1976 Feb;125(2):713–718. doi: 10.1128/jb.125.2.713-718.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Meynell E., Datta N. Mutant drug resistant factors of high transmissibility. Nature. 1967 May 27;214(5091):885–887. doi: 10.1038/214885a0. [DOI] [PubMed] [Google Scholar]
  10. Murray K., Murray N. E. Phage lambda receptor chromosomes for DNA fragments made with restriction endonuclease III of Haemophilus influenzae and restriction endonuclease I of Escherichia coli. J Mol Biol. 1975 Nov 5;98(3):551–564. doi: 10.1016/s0022-2836(75)80086-6. [DOI] [PubMed] [Google Scholar]
  11. Petrocheilou V., Grinsted J., Richmond M. H. R-plasmid transfer in vivo in the absence of antibiotic selection pressure. Antimicrob Agents Chemother. 1976 Oct;10(4):753–761. doi: 10.1128/aac.10.4.753. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. STRAUSS J. H., Jr, SINSHEIMER R. L. Purification and properties of bacteriophage MS2 and of its ribonucleic acid. J Mol Biol. 1963 Jul;7:43–54. doi: 10.1016/s0022-2836(63)80017-0. [DOI] [PubMed] [Google Scholar]
  13. Thomas M., Davis R. W. Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease. J Mol Biol. 1975 Jan 25;91(3):315–328. doi: 10.1016/0022-2836(75)90383-6. [DOI] [PubMed] [Google Scholar]
  14. Valtonen M. V., Valtonen V. V., Salo O. P., Mäkelä P. H. The effect of long term tetracycline treatment for acne vulgaris on the occurrence of R factors in the intestinal flora of man. Br J Dermatol. 1976 Sep;95(3):311–316. doi: 10.1111/j.1365-2133.1976.tb07019.x. [DOI] [PubMed] [Google Scholar]
  15. Wiedemann B., Knothe H. Untersuchungen über die Stabilität der Koliflora des gesunden Menschen. 1. Uber das Vorkommen permanenter und passanter Typen. Arch Hyg Bakteriol. 1969 Aug;153(4):342–348. [PubMed] [Google Scholar]
  16. Witchitz J. L., Chabbert Y. A. Résistance transférable à la gentamicine I. Expression du caractère de résistance. Ann Inst Pasteur (Paris) 1971 Dec;121(6):733–742. [PubMed] [Google Scholar]

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