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. 1977 Sep;12(3):368–372. doi: 10.1128/aac.12.3.368

Function of the Outer Membrane of Escherichia coli as a Permeability Barrier to Beta-Lactam Antibiotics

Willy Zimmermann 1, Armel Rosselet 1,†
PMCID: PMC429920  PMID: 334063

Abstract

On the basis of a simple theoretical model, the ease of penetration of β-lactam antibiotics through the outer membrane of Escherichia coli was measured. The cell envelope was found to act as a diffusion barrier to both penicillins and cephalosporins. The validity of the model and the cooperative action of cell-bound β-lactamase and outer membrane were further verified by comparing calculated and experimentally determined velocities of β-lactam hydrolysis by intact cells and sonically treated cell suspensions. The results showed good correspondence at five different antibiotic concentrations. Similar conclusions could be drawn from a comparison of β-lactam concentrations on both sides of the outer membrane, calculated from enzyme kinetic measurements and minimal inhibitory concentrations for both a β-lactamase-producing E. coli and its enzyme-negative variant. in the case of benzylpenicillin and cephalothin, however, no correspondence was found. The joint action of several parameters determining the efficacy of penicillins and cephalosporins against β-lactamase-producing E. coli is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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