Abstract
Monitoring response to chemo- or radiotherapy is of great importance in clinical practice. Apoptosis imaging serves as a very useful tool for the early evaluation of tumor response. The goal of this study was PET imaging of apoptosis with 18F-labeled recombinant human annexin V linked with 10 histidine tag (18F-rh-His10-annexin V) in nude mice bearing an A549 tumor and rabbits bearing a VX2 lung cancer after paclitaxel therapy. 18F-rh-His10-annexin V was prepared by conjugation of rh-His10-annexin V with N-succinimidyl 4-[18F]fluorobenzoate. Biodistribution was determined in mice by the dissection method and small-animal PET. Single-dose paclitaxel (175 mg/m2) was used to induce apoptosis in A549 and VX2 tumor models. 18F-rh-His10-annexin V was injected into A549 mice and VX rabbits to acquire dynamic and static PET images 72 h after paclitaxel treatment. The uptake of 18F-rh-His10-annexin V in apoptotic cells 4 h after induction was 6.45±0.52 fold higher than that in non-induced cells. High focal uptake of 18F-rh-His10-annexin V was visualized in A549 (SUVmax: 0.35±0.13) and VX2 (0.41±0.23) tumor models after paclitaxel treatment, whereas lower uptake was found in the corresponding tumors before treatment (A549 SUVmax: 0.04±0.02; VX2: 0.009±0.002). The apoptotic index was 75.61±11.56% in the treated VX2 cancer, much higher than that in the untreated VX2 (8.03±2.81%). This study demonstrated the feasibility of 18F-rh-His10-annexin V for the detection of apoptosis after chemotherapy in A549 and VX2 tumor models.
Keywords: Apoptosis, molecular imaging, recombinant human His10-annexin V, tumor response
Introduction
Detection of the early responses of tumors to treatment is critical for guiding subsequent therapy, allowing rapid selection of the most appropriate therapy. Tumor response to treatment is conventionally assessed by measuring tumor size with anatomical imaging such as CT or MRI [1]. However, there are several fundamental and practical limitations using purely anatomic measurements as a survival proxy. Tumor shrinkage can take weeks or even months to become apparent or, with some therapies, might not occur at all, despite a positive response to treatment [2]. 18F-FDG PET is well established in the clinical diagnosis, staging and management of malignant tumors [3,4], and has been used for early evaluation of tumor therapy response in many varieties of malignant tumor [5-9]. Persistently focal uptake of FDG after therapy always indicates a poor response and prognosis. However, FDG uptake is influenced by various factors, including blood glucose level, tumor sizes, inflammation, and acquisition protocol. Furthermore, slowly-growing, metabolically less active tumors including thyroid and prostate cancer and neuroendocrine tumor are not sensitive to FDG PET.
Apoptosis is the most common pathway for therapy-induced cancer cell elimination and lack of apoptosis is usually a sign of therapy failure [10,11]. Non-invasive molecular imaging of apoptosis is of considerable interest for assessing the therapy outcome and evaluating disease progression [12,13]. In the past decade, annexin V binding has become the main measurement of apoptosis in histopathology. To detect apoptosis after chemo- or radiotherapy, in vivo imaging with 99mTc-labeled annexin V and analogs has been performed and is well documented [14-24]. Our prior study showed that apoptosis imaging can monitor the response of non-small cell lung tumor graft to paclitaxel therapy. A positive correlation was shown between the uptake of radioactivity and TUNEL staining in the tumor areas [25]. Our latest study showed that 99mTc-rh-His10-annexin V can detect apoptosis in a porcine model with ischemia and reperfusion [26]. However, most of the 99mTc-labeled annexin V tracers displayed high uptake in the liver and kidney, which hampers the clinical application [27,28].
Because PET has higher resolution and is more quantitative than SPECT imaging with a 99mTc-labeled tracer, 18F-labeled annexin V has been developed and used for imaging apoptosis by many research groups. 18F-annexin V showed lower uptake in the liver and abdomen than 99mTc-annexin V, and could detect apoptosis in myocardial reperfusion injury and in the liver with cycloheximide treatment [29-32].
The current study aims to evaluate 18F-rh-His10-annexin V as a PET tracer for detecting apoptosis and early assessment of the outcome of paclitaxel chemotherapy in A549 tumor-bearing mouse and VX2 lung cancer-bearing rabbit models. In this study, we used recombinant human annexin V linked with a ten histidine tag (rh-His10-annexin V) at the N terminal of annexin V. This histidine tag simplified the purification of annexin V protein by ion exchange chromatography. The present study describes data about 18F-rh-His10-annexin V for the detection of apoptosis from a nude mice graft tumor model to rabbit VX2 tumor.
Materials and methods
General
18F was produced by the 18O (p, n) 18F nuclear reaction using a CYPRIS HM-18 cyclotron (Sumitomo Heavy Industry, Tokyo). If not otherwise stated, radioactivity was determined with an IGC-3R Curiemeter (Aloka, Tokyo). HPLC was performed using a JASCO HPLC system (JASCO, Tokyo): effluent radioactivity was monitored using a NaI (Tl) scintillation detector system. If not indicated, all chemical reagents of the highest grade commercially available were purchased from Sigma-Aldrich (St. Louis, MO) and Wako Pure Chem. Ind. (Osaka, Japan).
Expression and purification of rh-His10-annexin V
Human annexin V cDNA was cloned into plasmid pET19b and fused to a ten consecutive histidine tag at N-terminal [33]. By ammonium sulfate precipitation and two types of ion exchange chromatography, rh-His10-annexin V was purified to homogeneity with purity over 97%.
Synthesis of 18F-rh-His10-annexin V
[18F]F- was produced by the 18O (p, n) 18F reaction on 95 atom % H2 18O from the cyclotron. [18F]F- in aqueous K2CO3 (3.3 mg/0.3 mL) was transported into a vial containing CH3CN (1.5 mL)/Kryptofix 222 (15 mg) in a hot cell and was dried at 110°C for 15 min. To the dried [18F]F- was added a solution of 10 mg ethyl 4-(trimethylammonium triflate) benzoate in 0.2 mL anhydrous DMSO. This reaction mixture was heated at 110°C for 15 min. Hydrolysis of benzoate was performed by heating the mixture with 0.5 mL 1 N NaOH at 95°C for 10 min. After the mixture had been cooled for 2 min, 0.8 mL 1 N HCl was added. The reaction mixture was diluted to final volume of 15 mL with distilled water and loaded onto a Waters Sep-Pak cartridge that was activated in advance using 30 mL CH3CN and 30 mL distilled water. The loaded cartridge was washed with 2 mL 0.01 N HCl and dried for 2 min with a nitrogen stream. The radioactive fraction containing 4-18F-fluorobenzoic acid was eluted with 1.5 mL CH3CN.
The above radioactive fraction was heated with 20 μL 10% aqueous (CH3)4NOH solution for 15 min. After CH3CN had been removed with a nitrogen stream at 90°C, 1.5 mL anhydrous CH3CN was added and removed again. A solution of O-(N-succinimidyl)-N,N,N’,N’-tetramethyluronium tetrafluoroborate (15 mg) in 1 mL CH3CN was added and the reaction mixture was heated at 90°C for 5 min. This mixture was concentrated to 0.2 mL, cooled down, and diluted with 0.2 mL 5% aqueous acetic acid. HPLC semi-preparative purification of the mixture was performed on a YMC J’sphere ODS-H80 column (10 mm internal diameter ×250 mm) using a mobile phase of CH3CN/H2O (60/40) at 4.0 mL/min. The fraction of N-succinimidyl 4-18F-fluorobenzoate (18F-SFB, retention time: 10.5 min) was collected, diluted with 10 mL distilled water, and loaded to a Waters Sep-Pak cartridge. This cartridge was dried with a nitrogen stream and eluted with 2.5 mL CH3CN. The CH3CN eluent containing 18F-SFB was collected and evaporated to dryness under a gentle nitrogen stream at room temperature. The synthesis scheme was shown in (Figure 1A)
A solution of 18F-SFB in 0.2 M borate buffer pH 8.4 (50 μL) was added to a solution of rh-His10-annexin V (200 μg) in borate buffer pH 8.4 (50 μL). The mixture was kept at room temperature for 20 min. The reaction mixture was purified with size-exclusion column chromatography using Bio-Gel P-6 (BIO-RAD) according to the standard procedure.
Radiochemical purity determination
Radiochemical purity was assayed by analytical HPLC (Bio-Rad HPLC Gel Filtration, Bio-Sil EC-125, 7.8 mm 300 mm; 0.05 M NaH2PO4/0.05 M Na2HPO4/0.2 M NaCl: 1/1/1). The retention time for 18F-rh-His10-annexin V was 6.3 min at 1.0 mL/min.
SDS PAGE
SDS-PAGE was performed with a Mini-PROTEIN and POWER PAC 300 (Bio-Rad, Hercules, CA). Laemmli sample buffer (Bio-Rad) was added to each sample (1/1) and applied to Ready-gels J (Bio-Rad). Precision Protein Standard (Bio-Rad) was used as a standard. Electrophoresis was performed under constant voltage (200 V). After electrophoresis, gels were exposed to an imaging plate and radioactivity was analyzed by an imaging system (BAS5000, Fuji Photo Film, Tokyo, Japan), followed by staining with Bio-Safe Coomassie.
In vitro binding assays
Apoptosis was induced in Jurkat cells (RIKEN Cell Bank, Wako, Japan) at a density of 5×106 cells/mL using 3.5 μmol/L camptothecin (Sigma-Aldrich) for 4 h and 6 h. About 74 KBq of 18F-rh-His10-annexin V was incubated with 500 μL apoptotic or untreated cells (5×105) in 15 mM N-(2-hydroxythyl)piperazine-N-9-(2-ethanesulfonic acid), 120 mM NaCl and 2 mM CaCl2, pH 7.4, respectively. To separate binding cells from free 18F-rh-His10-annexin V, the mixture was centrifuged at 1,000 g for 2 min. After removing the aqueous supernatant, the left radioactivity was measured with a 1480 Wizard gamma counter (Perkin-Elmer, Walthan, MA).
Biodistribution study in mice
Male ddY mice (20~30 g, 7 weeks old; SLC, Shizuoka, Japan) were used. Each mouse was injected with 18F-rh-His10-annexin V (0.37 MBq/200 μL) via the tail vein. Three mice were sacrificed by cervical dislocation at 15 min, 60 min and 120 min after injection. Whole brain, liver, heart, lung, stomach, kidney, spleen, intestine and blood sample were removed quickly. Radio-activity was measured using a 1480 Wizard autogamma scintillation counter (Perkin-Elmer) and expressed as a percent of the injected dose per gram tissue (% ID/g) with the mean±standard deviations. All radioactivity was corrected for decay.
Metabolite analysis
After intravenous injection of 18F-rh-His10-annexin V (2.14 MBq/200 μL) in ddY mice, these mice were sacrificed by cervical dislocation at 120 min. Blood samples were acquired immediately and centrifuged at 15000 rpm for 1 min at 4°C, which of 250 μL plasma was acquired after centrifuge. At the same time point, 100 μL urine was collected. Fifty microliter of plasma and urine were analyzed by HPLC with the same parameters for the determination of radiochemical purity. The ratio of 18F-rh-His10-annexin V o total radioactivity on the HPLC chromatogram was analyzed and calculated as a percentage.
In vivo imaging with small-animal PET
PET scan was performed using a small-animal PET Inveon scanner (Siemens Medical Solutions USA). Prior to the scans, a mouse was anesthetized with 1.5% (v/v) isoflurane and positioned on the scanner bed. After transmission scans for attenuation correction were performed for 2 cycles (803 sec) using a 57Co point source, dynamic emission scans were acquired for 120 min in a 3D list mode with an energy window of 350~650 keV, immediately after intravenous injection of 1.85 MBq [18F]rh-His10-annexin V. All list-mode data were sorted into 3D sinograms, which were then Fourier rebinned into 2D sinograms (frames: 4×1, 8×2 and 20×5 min). Dynamic images were reconstructed with filtered back-projection using a Ramp filter. For data analysis, region of interest (ROI) were drawn on lung, heart, liver, and kidney using ASIPro VM (Analysis Tools and System Setup/Diagnostics Tool), the time-radioactivity curve, Standard uptake value (SUV) and ID%/g were acquired automatically. SUVmax was calculated by measuring the maximal concentration of radioactivity in a ROI and correcting it for body weight and injected dose (SUVmax=maximum activity concentration/[injected dose/body weight]) in A549 tumor model.
Preparation of A549 nude mice tumor model and VX2 rabbit lung tumor model
A549 tumor cells were cultured as a monolayer in Dulbecco’s modified Eagle’s F-12 medium supplemented with 10% fetal bovine serum. Female nude mice (BALB/c) weighing 18~22 g were inoculated subcutaneously in the anterior chest wall with an average 5×106 A549 tumor cells per site. When the tumor size reached 0.5-1.0 cm in diameter, which required about 2 weeks, the mice were separated into 2 groups (5 mice per each group). One group received paclitaxel (175 mg/m2) by injection into the tail vein, another without treatment served as control.
Eight New Zealand white rabbits (Nanjing Medical University) weighing 2~3 kg were used. VX2 tumor is the biggest tumor model, which closely resembles human cancer. VX2 cells were initially grown in the hind limb of a donor rabbit. Each rabbit received general anesthesia with 3% pentobarbital (30 mg/kg) through an indwelling catheter in the auricular vein. VX2 tumor was surgically removed from a donor rabbit under general anesthesia and minced into 1-mm3 pieces with a pair of scissors. After receiving anesthesia, two 1.0-cm-deep tunnels were made bilaterally into the skin of the front chest, and one or two 1-mm3 pieces of VX2 tissue were implanted into each tunnel. The incisions were closed with 3.0 sutures. When the tumors had grown to approximately 1~2 cm in diameter, the rabbit was used in the experiment. Six VX2 tumor rabbits received a single dose of paclitaxel (175 mg/m2), two served as control.
Image interpretation in A549 tumor model
After 18F-rh-His10-annexin V (3.7 MBq) injected via the tail vein, a dynamic scan was performed for 120 min after injection and static images were acquired from 105 min to 120 min. PET scans were performed in mice with A549 before and after paclitaxel treatment. For data analysis, ROIs were drawn on the tumor and peripheral organs using ASIPro VM.
After imaging, the mice were scarified. In the A549 tumor model, hematoxylin and eo-sin (H&E) staining was performed to verify apoptosis occurrence in the frozen sections. DNA fragmentation was analyzed by terminal deoxynucleotidytransferase mediated-dUTP nick-end labeling (TUNEL) assay using an ApopTag peroxidase in situ oligonucleotide (oligo) ligation (ISOL) apoptosis detection kit (S7200; Chemicon/Millipore, Billerica, MA) according to the manufacturer’s protocol.
Imaging and interpretation in VX2 tumor model
Six rabbits bearing a VX2 tumor were treated by intravenous injection of paclitaxel at a dose of 175 mg/m2. Before and 72 h after treatment, 18F-rh-His10-annexin V (18.5~37 MBq) was injected via the auricular vein, PET-CT (Philips Medical System) was performed 60 min after injection. Two of the VX2 tumor model rabbits served as a control and did not receive treatment. PET scans with 18F-FDG (37 MBq) were performed using the same rabbits. Each PET/CT scan was performed 60 min after injection. Emission data were acquired at 3 min per bed position and CT component was performed using a multi-detector scanner before the emission component. The parameters included 140 kV, 80 mA, 0.8 s per CT rotation, a pitch of 5.0 mm, and a table speed of 22.5 mm/s. Images were analyzed visually and quantified with SUVmax based on attenuation-corrected images.
After imaging, the rabbits were sacrificed and formalin-fixed paraffin-embedded tumor tissues were excised after imaging. DNA fragmentation were analyzed by TUNEL assay using a commercial kit (Maxin-Bio) according the manufacturer’s protocol. In random fields, TUNEL-positive nuclei as a percentage of total nuclei was determined and used as the apoptotic index.
Statistical analysis
All data are expressed as the mean±SD, statistical analysis was performed using SPSS 10.0, t tests were used to compare the treated group and untreated controls, p < 0.05 was considered significant.
Results
Synthesis of 18F-rh-His10-annexin V
The decay corrected radiochemical yield of 18F-rh-His10-annexin V was 20~25% based on 18F-SFB with a synthesis time of 90 min from the end of bombardment. At the end of synthesis, 130~160 MBq of 18F-rh-His10-annexin V was obtained as an i.v. injectable solution with a radiochemical purity of > 98% determined by HPLC. The autoradiograph and Coomassie staining of polyacrylamide after electrophoresis further verified the radiochemically pure product (Figure 1B), no degradation was observed during radiolabeling and synthesis.
In vitro binding assay
The binding specificity of 18F-rh-His10-annexin V was evaluated with camptothecin-induced Jurkat cells. After inducement, apoptotic cells was observed at 4 h and 6 h. Incubation with 18F-rh-His10-annexin V showed that the uptake of radioactivity in the induced cells was 6.45±0.52 fold and 8.12±0.45 fold higher than that of the untreated cells (p < 0.01), respectively (Figure 2).
Biodistribution on mice
The biodistrbution profile of 18F-rh-His10-annexin V uptake in normal mice was shown in (Figure 3A, Table 1). The most predominant uptake was shown in the kidney, and the radioactivity decreased rapidly to less than 10% of the maximum level 120 min after injection. At 15 min after injection, lower uptake was found in nonspecific peripheral organs, such as the heart, gastrointestinal tract and skeletal muscles. Rapid clearance of radioactivity was observed in blood, the uptake value was 0.28±0.04 ID%/g at 120 min after injection.
Table 1.
Organs | 15 min | 60 min | 120 min |
---|---|---|---|
Blood | 2.00±0.85 | 1.12±0.25 | 0.28±0.04 |
Heart | 1.31±0.45 | 0.91±0.26 | 0.37±0.03 |
Liver | 3.89±0.90 | 1.48±0.18 | 0.36±0.07 |
Spleen | 5.03±1.55 | 2.24±1.54 | 1.09±0.07 |
Lung | 7.89±1.70 | 1.54±1.04 | 0.81±0.31 |
Kidney | 35.43±19.46 | 13.75±2.41 | 3.56±0.22 |
Intestine | 0.81±0.33 | 2.52±0.60 | 0.98±0.18 |
Large bowel | 0.34±0.15 | 1.05±0.85 | 1.56±0.39 |
Muscle | 0.39±0.13 | 0.51±0.08 | 0.17±0.03 |
Brain | 0.09±0.04 | 0.05±0.01 | 0.02±0.01 |
Stomach | 1.08±0.77 | 4.70±3.55 | 0.42±0.14 |
The radioactivity was expressed as ID %/g (n=3).
Time-radioactivity curves in the main organs are shown in (Figure 3B). The initial high uptake was visualized in the liver and heart, which represented the uptake of blood pool. The liver uptake peaked at 1.5 min after injection, decreased rapidly to a low level. The kidney uptake reached the maximum at 20 min and then decreased until the end of PET scan.
Metabolite analysis
Metabolite analysis of 18F-rh-His10-annexin V was performed for mouse plasma and urine. At 120 min after injection, about 60% of total radioactivity represented the unchanged 18F-rh-His10-annexin V in the plasma. On the other hand, 74% of total radioactivity in the urine represented the radiolabeled metabolites at 120 min.
PET imaging for mice and histopathology
High focal uptake of 18F-rh-His10-annexin V was visualized in the A549 tumor after treatment by paclitaxel (Figure 4A, the Middle and Right image), whereas lower uptake was seen A549 mice before treatment (Figure 4A, Left image). The SUVmax in tumor after treatment was 0.35±0.13, significantly higher than the group before treatment (0.04±0.02, P < 0.001). After paclitaxel treatment, a large number of apoptotic cells were observed. H&E and TUNEL staining showed that the apoptotic cells increased in the treated group compared to the untreated group (Figure 4B).
PET imaging for rabbits and histopathology
Representative images of VX2 rabbit lung cancer were shown in (Figure 5) 18F-FDG image showed high focal uptake in the primary tumor before treatment (Figure 5A), whereas no significant uptake of 18F-rh-His10-annexin V was found in FDG avid foci. After paclitaxel treatment, 18F-rh-His10-annexin V images showed intense uptake in the tumor (Figure 5B). By analyzing the images of treatment and the untreated controls, the SUVmax of 18F-rh-His10-annexin V after treatment was 0.41±0.23, that of the untreated group was 0.009±0.002 (t=0.78, P < 0.001).
Histological analysis showed a small number of apoptotic cells were found before treatment, which were characterized by cell shrinkage, condensed nuclei, and the appearance of apoptotic bodies, after the treatment, a large number of apoptotic cells were observed (Figure 6). TUNEL-positive nuclei confirmed the DNA fragmentation. The apoptotic index was 75.61±11.56% in the treated group, significantly higher than that in the control (8.03±2.81%; P < 0.001).
Discussion
18F-rh-His10-annexin V was prepared by labeling rh-His10-annexin V with 18F-SFB. Radiolabeling was reproducible with 20~35% radiochemical yield based on 18F-SFB. After size-column exclusion chromatography, the radiochemical purity of this 18F-labeled radiotracer exceeded 95%. Further, the radiochemical purity remained > 90% after standing the product at 25°C for 3 h. No degradation of protein was found during the synthesis and maintaining processes. This result indicates radiochemical stability of 18F-rh-His10-annexin V within the time of at least one PET scan. The analytical results were in compliance with our in-house quality control/assurance specifications of radiopharmaceuticals.
18F-rh-His10-annexin V can bind to apoptotic Jurkat cells induced by camptothecin, and the uptake in the induced cells was higher than that of untreated cells. Despite the introduction of a ten histidine tag, the specific binding of this protein to apoptotic cells was not affected. The in vitro results confirmed that 18F-rh-His10-annexin V was worth in vivo evaluation and had shown its usefulness.
18F-rh-His10-annexin V showed a superior biodistribution profile compared with 99mTc-labeled-annexin V [27-28]. PET dynamic data further confirmed that it mainly excreted from kidney and urinary system, rapid clearance from blood and liver was observed. This finding indicates that the 18F-labeled tracer may have more benefits for the detection of apoptosis than 99mTc-labeled-annexin V, which usually showed slow excretory clearance and relatively high uptake in the liver and spleen. On the other hand, metabolite analysis showed a high percentage of radiolabeled metabolite in the mouse urine at 120 min after injection of 18F-rh-His10-annexin V, which may contribute to the lower uptake of radioactivity in the kidney.
In nude mice bearing A549 tumor, focal uptake was visualized in the tumor after paclitaxel treatment. VX2 is the biggest tumor model and closely imitate human lung cancer initiation, development and progress. In VX2 lung cancer, as shown in Figure 5, multiple FDG avid foci was found before treatment, while high uptake of 18F-rh-His10-annexin V was shown in the tumor 72 h after treatment, the SUVmax in the tumor after therapy was much higher than in the untreated group. A large number of TUNEL-positive nuclei were observed after treatment. H&E staining also revealed the presence of a large number of apoptotic cells associated with cell shrinkage, condensed nuclei and apoptotic bodies. Only a small number of apoptotic cells were confirmed in the control. This finding revealed that increased accumulation of 18F-rh-His10-annexin V in the tumor after paclitaxel treatment was caused by ongoing apoptosis. The present PET study with 18F-rh-His10-annexin V showed that this radiotracer is feasible to detect apoptosis after chemotherapy not only in A549 nude mice tumor model but in VX2 lung cancer as a mimic of human lung cancer.
Conclusions
This study demonstrated the feasibility of 18F-rh-His10-annexin V for the detection of apoptosis after chemotherapy from A549 graft to VX2 lung tumor models which could imitate the development and progress of human cancer. The current study will serve as a reference point that gauges the early tumor therapy outcome with apoptosis imaging.
Acknowledgements
We thank the staff of the National Institute of Radiological Sciences for support in the cyclotron operation, radioisotope production, and animal experiments. This research was supported by the Jiangsu Provincial Nature Science Foundation (BL2012037, BK2011104, BK2006010), the Chinese National Nature Sciences Foundation (81271604, 81171383).
Disclosure of conflict of interest
The authors have no conflict of interest.
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