Abstract
Here, we report the draft genome of Robinsoniella peoriensis strain WTD, which was isolated from the fecal material of a wood turtle. The genome size was 7,391,415 bp with 41.1 mol% G+C.
GENOME ANNOUNCEMENT
Robinsoniella peoriensis was identified in 2009 as an anaerobic Gram-positive rod that is capable of forming endospores. It was originally isolated from a swine-manure storage pit (1, 2). In humans, there have been several reported cases of R. peoriensis infection (3–7). In this study, R. peoriensis strain WTD was isolated from the fecal material of a wood turtle using the ethanol shock method (8). Cells were plated on blood agar and incubated for 48 h under anaerobic conditions.
A colony of R. peoriensis was sent to Molecular Research LP (MR DNA; http://www.mrdnalab.com). DNA was isolated using the Qiagen mini DNA isolation kit (Qiagen Inc.). Afterward, using an Ion Express library kit and the Ion Torrent PGM sequencer (Life Technologies), a draft genome was sequenced following the manufacturer’s guidelines. Using NGEN (DNAstar), the genome was assembled and annotation of the genome was performed using RAST (http://rast.nmpdr.org) (9).
In total, 32 contigs with protein-encoding genes were constructed, which totaled 7,391,415 bp with a G+C content of 41.1%. Annotation in RAST revealed 73 RNAs, 6,115 coding sequences, and 370 subsystems. There were 129 genes involved in virulence, disease, and defense. Some of these genes are involved in vancomycin, fluoroquinolones, and tetracycline resistance.
The availability of this draft genome may aid in determining the role of this bacterium in human illnesses.
Nucleotide sequence accession numbers.
This whole-genome shotgun project has been deposited at DDBJ/EMBL/GenBank under the accession number JTGN00000000. The version described in this paper is version JTGN01000000.
ACKNOWLEDGMENTS
This research was done as part of a Provost Honors project under the leadership of Zina Haywood, Executive Vice President/Provost. We also thank Kathy Nordhaus and Donald Zakutansky for their enthusiastic support of this research. This project was supported by funding provided by Gateway Technical College and by the Gateway Foundation.
Footnotes
Citation Braasch JL, Lapin CN, Dowd SE, McLaughlin RW. 2015. Draft genome sequence of Robinsoniella peoriensis strain WTD, isolated from the fecal material of a wood turtle. Genome Announc 3(1):e01444-14. doi: 10.1128/genomeA.01444-14.
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