Abstract
A new assay was developed to determine the concentration of amphotericin B in body fluids. The Bactec radiometric system was used to measure CO2 production by a test strain, Candida albicans MDA 448, in the presence of amphotericin B. After 5-h incubation, drug concentrations as low as 0.2 μg/ml could be detected. The results are comparable to those of the commonly used agar diffusion assay with Paecilomyces varioti.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bindschadler D. D., Bennett J. E. A pharmacologic guide to the clinical use of amphotericin B. J Infect Dis. 1969 Oct;120(4):427–436. doi: 10.1093/infdis/120.4.427. [DOI] [PubMed] [Google Scholar]
- Calam D. H. Chromatographic analysis of heptaene macrolide antibiotics. J Chromatogr Sci. 1974 Oct;12(10):613–616. doi: 10.1093/chromsci/12.10.613. [DOI] [PubMed] [Google Scholar]
- Drazin R. E., Lehrer R. I. Rubidium release: a rapid and sensitive assay for amphotericin B. J Infect Dis. 1976 Sep;134(3):238–244. doi: 10.1093/infdis/134.3.238. [DOI] [PubMed] [Google Scholar]
- Herd P. A., Martin H. F. A semi-quantitative spectrophotometric assay for amphotericin B in blood. Clin Biochem. 1974 Dec;7(4):359–365. doi: 10.1016/s0009-9120(74)92900-2. [DOI] [PubMed] [Google Scholar]
- Hopfer R. L., Gröschel D. Amphotericin B susceptibility testing of yeasts with a bactec radiometric system. Antimicrob Agents Chemother. 1977 Feb;11(2):277–280. doi: 10.1128/aac.11.2.277. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Polak A., Scholer H. J. Fungistatic activity, uptake and incorporation of 5-fluorocytosine in Candida albicans, as influenced by pyrimidines and purines. I. Reversal experiments. Pathol Microbiol (Basel) 1973;39(2):148–159. doi: 10.1159/000162642. [DOI] [PubMed] [Google Scholar]
- Shadomy S., McCay J. A., Schwartz S. I. Bioassay for hamycin and amphotericin B in serum and other biological fluids. Appl Microbiol. 1969 Apr;17(4):497–503. doi: 10.1128/am.17.4.497-503.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thomas A. H. Analysis and assay of polyene antifungal antibiotics. A review. Analyst. 1976 May;101(1202):321–340. doi: 10.1039/an9760100321. [DOI] [PubMed] [Google Scholar]