Table 1.
Melting temperatures (in °C; means ± standard deviation) of SmTAL1 (5 μM) and SmTAL3 (7 μM) (determined by DSF) under various conditions.
| SmTAL1 | SmTAL3 | |
|---|---|---|
| EGTA (0.4 mM) | 58.4 ± 0.5 | 73.6 ± 0.4 |
| EGTA (0.4 mM)/CaCl2 (0.8 mM) | 65.7 ± 0.7a | 73.6 ± 0.4 |
| DMSO (1% v/v) | 60.3 ± 0.1 | 74.6 ± 0.1 |
| Praziquantel (0.25 mM) | 61.7 ± 0.2b | 74.5 ± 0.0 |
| Chlorpromazine (0.25 mM) | 61.3 ± 0.3b | 74.1 ± 0.1b |
| W7 (0.25 mM) | 61.5 ± 0.3b | 74.3 ± 0.1b |
| Trifluoperazine (0.25 mM) | 62.2 ± 0.8b | 73.9 ± 0.1b |
| Thiamylal (0.25 mM) | 60.5 ± 0.7 | 74.5 ± 0.4 |
a
Significantly different (p < 0.05 in an unpaired t-test with Welch's correction) from the value for the same protein in EGTA only.
b
Significantly different (p < 0.05 in an unpaired t-test with Welch's correction) from the value for the same protein in the presence of DMSO. In the experiments with drugs, the final concentration of DMSO was 1% v/v. The DMSO control and the experiments with drugs all contain 0.8 mM calcium chloride.