Abstract
The DNAs from five independent simian virus 40 (SV40) transformants of the BALB/c3T3 mouse cell line were digested with either the HpaII or the BamHI restriction endonuclease and the resulting fragments were fractionated by gel electrophoresis. The DNA fragments were denatured in situ in the gel and transferred to a membrane filter. Fragments containing viral DNA were detected by hybridization with high specific activity 32P-labeled SV40 complementary RNA (cRNA) synthesized in vitro. Each of the lines yielded a small number of fragments containing SV40 DNA and the fragments from each line were different. This observation shows that the structure of the integrated SV40 DNA and/or its location in the host DNA are different in each line.
Full text
PDF![1102](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd85/430208/b78c4fd204ba/pnas00034-0128.png)
![1103](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd85/430208/363a80c0757d/pnas00034-0129.png)
![1104](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd85/430208/e96e9d50d9c0/pnas00034-0130.png)
![1105](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd85/430208/cee07d5e6ace/pnas00034-0131.png)
![1106](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd85/430208/a848a426b2af/pnas00034-0132.png)
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bader J. P. Temperature-dependent transformation of cells infected with a mutant of Bryan Rous sarcoma virus. J Virol. 1972 Aug;10(2):267–276. doi: 10.1128/jvi.10.2.267-276.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Benjamin T. L. Virus-specific RNA in cells productively infected or transformed by polyoma virus. J Mol Biol. 1966 Apr;16(2):359–373. doi: 10.1016/s0022-2836(66)80179-1. [DOI] [PubMed] [Google Scholar]
- Biquard J. M., Vigier P. Characteristics of a conditional mutant of Rous sarcoma virus defective in ability to transform cells at high temperature. Virology. 1972 Feb;47(2):444–455. doi: 10.1016/0042-6822(72)90280-2. [DOI] [PubMed] [Google Scholar]
- Biquard J. M., Vigier P. Isolement et étude d'un mutant conditionnel du virus de Rous à capacité transformante thermosensible. C R Acad Sci Hebd Seances Acad Sci D. 1970 Dec 21;271(25):2430–2433. [PubMed] [Google Scholar]
- Botchan M., McKenna G., Sharp P. A. Cleavage of mouse DNA by a restriction enzyme as a clue to the arrangement of genes. Cold Spring Harb Symp Quant Biol. 1974;38:383–395. doi: 10.1101/sqb.1974.038.01.041. [DOI] [PubMed] [Google Scholar]
- Brugge J. S., Butel J. S. Role of simian virus 40 gene A function in maintenance of transformation. J Virol. 1975 Mar;15(3):619–635. doi: 10.1128/jvi.15.3.619-635.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chow L. T., Boyer H. W., Tischer E. G., Goodman H. M. Electron microscopic mapping of the attachment sites on SV40 DNA during lytic infection. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):109–117. doi: 10.1101/sqb.1974.039.01.016. [DOI] [PubMed] [Google Scholar]
- Croce C. M., Huebner K., Girardi A. J., Koprowski H. Genetics of cell transformation by simian virus 40. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):335–343. doi: 10.1101/sqb.1974.039.01.044. [DOI] [PubMed] [Google Scholar]
- Danna K., Nathans D. Specific cleavage of simian virus 40 DNA by restriction endonuclease of Hemophilus influenzae. Proc Natl Acad Sci U S A. 1971 Dec;68(12):2913–2917. doi: 10.1073/pnas.68.12.2913. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Garfin D. E., Goodman H. M. Nucleotide sequences at the cleavage sites of two restriction endonucleases from Hemophilus parainfluenzae. Biochem Biophys Res Commun. 1974 Jul 10;59(1):108–116. doi: 10.1016/s0006-291x(74)80181-6. [DOI] [PubMed] [Google Scholar]
- Gerber P. Studies on the transfer of subviral infectivity from SV40-induced hamster tumor cells to indicator cells. Virology. 1966 Apr;28(4):501–509. doi: 10.1016/0042-6822(66)90234-0. [DOI] [PubMed] [Google Scholar]
- Gillespie D., Spiegelman S. A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J Mol Biol. 1965 Jul;12(3):829–842. doi: 10.1016/s0022-2836(65)80331-x. [DOI] [PubMed] [Google Scholar]
- Helling R. B., Goodman H. M., Boyer H. W. Analysis of endonuclease R-EcoRI fragments of DNA from lambdoid bacteriophages and other viruses by agarose-gel electrophoresis. J Virol. 1974 Nov;14(5):1235–1244. doi: 10.1128/jvi.14.5.1235-1244.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. doi: 10.1016/0022-2836(67)90307-5. [DOI] [PubMed] [Google Scholar]
- Kawai S., Hanafusa H. The effects of reciprocal changes in temperature on the transformed state of cells infected with a rous sarcoma virus mutant. Virology. 1971 Nov;46(2):470–479. doi: 10.1016/0042-6822(71)90047-x. [DOI] [PubMed] [Google Scholar]
- Kawai S., Metroka C. E., Hanafusa H. Complementation of functions required for cell transformation by double infection with RSV mutants. Virology. 1972 Jul;49(1):302–304. doi: 10.1016/s0042-6822(72)80032-1. [DOI] [PubMed] [Google Scholar]
- Kelly T. J., Jr, Lewis A. M., Jr, Levine A. S., Siegel S. Structure of two adenovirus-simian virus 40 hybrids which contain the entire SV40 genome. J Mol Biol. 1974 Oct 15;89(1):113–126. doi: 10.1016/0022-2836(74)90165-x. [DOI] [PubMed] [Google Scholar]
- Kimura G., Itagaki A. Initiation and maintenance of cell transformation by simian virus 40: a viral genetic property. Proc Natl Acad Sci U S A. 1975 Feb;72(2):673–677. doi: 10.1073/pnas.72.2.673. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Koprowski H., Jensen F. C., Steplewski Z. Activation of production of infectious tumor virus SV40 in heterokaryon cultures. Proc Natl Acad Sci U S A. 1967 Jul;58(1):127–133. doi: 10.1073/pnas.58.1.127. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lee T. N., Brockman W. W., Nathans D. Evolutionary variants of simian virus 40: cloned substituted variants containing multiple initiation sites for DNA replication. Virology. 1975 Jul;66(1):53–69. doi: 10.1016/0042-6822(75)90178-6. [DOI] [PubMed] [Google Scholar]
- Manor H., Fogel M., Sachs L. Integration of viral into chromosomal deoxyribonucleic acid in an inducible line of polyoma-transformed cells. Virology. 1973 May;53(1):174–185. doi: 10.1016/0042-6822(73)90476-5. [DOI] [PubMed] [Google Scholar]
- Martin G. S. Rous sarcoma virus: a function required for the maintenance of the transformed state. Nature. 1970 Sep 5;227(5262):1021–1023. doi: 10.1038/2271021a0. [DOI] [PubMed] [Google Scholar]
- Martin R. G., Chou J. Y. Simian virus 40 functions required for the establishment and maintenance of malignant transformation. J Virol. 1975 Mar;15(3):599–612. doi: 10.1128/jvi.15.3.599-612.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nathans D., Smith H. O. Restriction endonucleases in the analysis and restructuring of dna molecules. Annu Rev Biochem. 1975;44:273–293. doi: 10.1146/annurev.bi.44.070175.001421. [DOI] [PubMed] [Google Scholar]
- Osborn M., Weber K. Simian virus 40 gene A function and maintenance of transformation. J Virol. 1975 Mar;15(3):636–644. doi: 10.1128/jvi.15.3.636-644.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reid M. S., Bieleski R. L. A simple apparatus for vertical flat-sheet polyacrylamide gel electrophoresis. Anal Biochem. 1968 Mar;22(3):374–381. doi: 10.1016/0003-2697(68)90278-9. [DOI] [PubMed] [Google Scholar]
- Sambrook J., Botchan M., Gallimore P., Ozanne B., Pettersson U., Williams J., Sharp P. A. Viral DNA sequences in cells transformed by simian virus 40, adenovirus type 2 and adenovirus type 5. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):615–632. doi: 10.1101/sqb.1974.039.01.075. [DOI] [PubMed] [Google Scholar]
- Sambrook J., Westphal H., Srinivasan P. R., Dulbecco R. The integrated state of viral DNA in SV40-transformed cells. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1288–1295. doi: 10.1073/pnas.60.4.1288. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sharp P. A., Sugden B., Sambrook J. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis. Biochemistry. 1973 Jul 31;12(16):3055–3063. doi: 10.1021/bi00740a018. [DOI] [PubMed] [Google Scholar]
- Shinnick T. M., Lund E., Smithies O., Blattner F. R. Hybridization of labeled RNA to DNA in agarose gels. Nucleic Acids Res. 1975 Oct;2(10):1911–1929. doi: 10.1093/nar/2.10.1911. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
- TEMIN H. M. THE PARTICIPATION OF DNA IN ROUS SARCOMA VIRUS PRODUCTION. Virology. 1964 Aug;23:486–494. doi: 10.1016/0042-6822(64)90232-6. [DOI] [PubMed] [Google Scholar]
- Tegtmeyer P. Function of simian virus 40 gene A in transforming infection. J Virol. 1975 Mar;15(3):613–618. doi: 10.1128/jvi.15.3.613-618.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tordaro G. J., Green H. An assay for cellular transformation by SV40. Virology. 1964 May;23(1):117–119. doi: 10.1016/s0042-6822(64)80018-0. [DOI] [PubMed] [Google Scholar]
- Tournier P., Cassingena R., Wicker R., Coppey J., Suarez H. Etude du mécanisme de l'induction chez des cellules de hamster syrien transformées par le virus SV40. I. Propriétés d'une lignée cellulaire clonale. Int J Cancer. 1967 Mar 15;2(2):117–132. doi: 10.1002/ijc.2910020207. [DOI] [PubMed] [Google Scholar]
- Varmus H. E., Bishop J. M., Vogt P. K. Appearance of virus-specific DNA in mammalian cells following transformation by Rous sarcoma virus. J Mol Biol. 1973 Mar 15;74(4):613–626. doi: 10.1016/0022-2836(73)90052-1. [DOI] [PubMed] [Google Scholar]
- Varmus H. E., Vogt P. K., Bishop J. M. Integration of deoxyribonucleic acid specific for Rous sarcoma virus after infection of permissive and nonpermissive hosts. Proc Natl Acad Sci U S A. 1973 Nov;70(11):3067–3071. doi: 10.1073/pnas.70.11.3067. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Watkins J. F., Dulbecco R. Production of SV40 virus in heterokaryons of transformed and susceptible cells. Proc Natl Acad Sci U S A. 1967 Oct;58(4):1396–1403. doi: 10.1073/pnas.58.4.1396. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Westphal H., Dulbecco R. Viral DNA in polyoma- and SV40-transformed cell lines. Proc Natl Acad Sci U S A. 1968 Apr;59(4):1158–1165. doi: 10.1073/pnas.59.4.1158. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Westphal H. SV40 DNA strand selection by Escherichia coli RNA polymerase. J Mol Biol. 1970 Jun 14;50(2):407–420. doi: 10.1016/0022-2836(70)90201-9. [DOI] [PubMed] [Google Scholar]
- Wilson G. A., Young F. E. Isolation of a sequence-specific endonuclease (BamI) from Bacillus amyloliquefaciens H. J Mol Biol. 1975 Sep 5;97(1):123–125. doi: 10.1016/s0022-2836(75)80028-3. [DOI] [PubMed] [Google Scholar]
- Wyke J. A. Complementation of transforming functions by temperature-sensitive mutants of avian sarcoma virus. Virology. 1973 Jul;54(1):28–36. doi: 10.1016/0042-6822(73)90111-6. [DOI] [PubMed] [Google Scholar]
- zur Hausen H. Oncogenic Herpes viruses. Biochim Biophys Acta. 1975 Mar 20;417(1):25–53. doi: 10.1016/0304-419x(75)90007-4. [DOI] [PubMed] [Google Scholar]