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. 1976 Apr;73(4):1107–1111. doi: 10.1073/pnas.73.4.1107

A 15-hydroxyprostaglandin dehydrogenase specific for prostaglandin A in rabbit kidney.

H G Oien, E A Ham, M E Zanetti, E H Ulm, F A Kuehl Jr
PMCID: PMC430209  PMID: 4794

Abstract

Examination of a soluble fraction derived from homogenates of rabbit kidney papilla revealed the existence of a 15-hydroxyprostaglandin dehydrogenase specific for A-type prostaglandins. Prostaglandins of the E- and F-series were not substrates for this enzyme. In agreement with published data, the 15-hydroxyprostaglandin dehydrogenase(s) derived from the kidney cortex were found to degrade all prostaglandins examined (PGE, PGF, PGA) in the presence of added cofactor NAD. Thus it is evident that in this species the kidney 15-hydroxyprostaglandin dehydrogenases are anatomically compartmentalized so that the papilla is able to metabpable of degrading E-, F-, and A-type prostaglandins by this metabolic pathway.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anders N. H. Preparative thin-layer and column chromatography of prostaglandins. J Lipid Res. 1969 May;10(3):316–319. [PubMed] [Google Scholar]
  2. Andersen N. H. Dehydration of prostaglandins: study by spectroscopic method. J Lipid Res. 1969 May;10(3):320–325. [PubMed] [Google Scholar]
  3. Attallah A. A., Payakkapan W., Lee J. B. Metabolism of prostaglandin A. I. The kidney cortex as a major site of PGA2 degradation. Life Sci. 1974 Apr 16;14(8):1521–1534. doi: 10.1016/0024-3205(74)90163-5. [DOI] [PubMed] [Google Scholar]
  4. GREEN K., SAMUELSSON B. SYNTHESIS OF TRITIUM-LABELED PROSTAGLANDIN E-2 AND STUDIES ON ITS METABOLISM IN GUINEA PIG LUNG. 37. PROSTAGLANDINS AND RELATED FACTORS. J Biol Chem. 1965 May;240:1932–1940. [PubMed] [Google Scholar]
  5. Ham E. A., Oien H. G., Ulm E. H., Kuehl F. A., Jr The reaction of PGA1 with sulfhydryl groups; a component in the binding of A-type prostaglandins to proteins. Prostaglandins. 1975 Aug;10(2):217–229. doi: 10.1016/0090-6980(75)90041-6. [DOI] [PubMed] [Google Scholar]
  6. Horton E. W., Jones R. L. Prostaglandins A1,A2 and 19-hydroxy A1; their actions on smooth muscle and their inactivation on passage through the pulmonary and hepatic portal vascular beds. Br J Pharmacol. 1969 Nov;37(3):705–722. doi: 10.1111/j.1476-5381.1969.tb08509.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Larsson C., Anggård E. Regional differences in the formation and metabolism of prostaglandins in the rabbit kidney. Eur J Pharmacol. 1973 Jan;21(1):30–36. doi: 10.1016/0014-2999(73)90202-1. [DOI] [PubMed] [Google Scholar]
  8. Lee S. C., Levine L. Prostaglandin metabolism. II. Identification of two 15-hydroxyprostaglandin dehydrogenase types. J Biol Chem. 1975 Jan 25;250(2):548–552. [PubMed] [Google Scholar]
  9. Matschinsky F. M., Shanahan D., Ellerman J. An improved procedure for the enzymatic analysis of prostaglandins in the nanogram range. Anal Biochem. 1974 Jul;60(1):188–199. doi: 10.1016/0003-2697(74)90144-4. [DOI] [PubMed] [Google Scholar]
  10. McGiff J. C., Terragno N. A., Strand J. C., Lee J. B., Lonigro A. J., Ng K. K. Selective passage of prostaglandins across the lung. Nature. 1969 Aug 16;223(5207):742–745. doi: 10.1038/223742b0. [DOI] [PubMed] [Google Scholar]
  11. Nakano J., Anggård E., Samuelsson B. 15-Hydroxy-prostanoate dehydrogenase. Prostaglandins as substrates and inhibitors. Eur J Biochem. 1969 Dec;11(2):386–389. doi: 10.1111/j.1432-1033.1969.tb00784.x. [DOI] [PubMed] [Google Scholar]
  12. Raz A. Interaction of prostaglandins with blood plasma proteins. Comparative binding of prostaglandins A 2 , F 2 and E 2 to human plasma proteins. Biochem J. 1972 Nov;130(2):631–636. doi: 10.1042/bj1300631. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. SAMUELSSON B. PROSTAGLANDINS AND RELATED FACTORS. 28. METABOLISM OF PROSTAGLANDIN E1 IN GUINEA PIG LUNG: THE STRUCTURES OF TWO METABOLITES. J Biol Chem. 1964 Dec;239:4097–4102. [PubMed] [Google Scholar]
  14. Saeed S. A., Roy A. C. Purification of 15-hydroxy prostaglandin dehydrogenase from bovine lung. Biochem Biophys Res Commun. 1972 Apr 14;47(1):96–102. doi: 10.1016/s0006-291x(72)80015-9. [DOI] [PubMed] [Google Scholar]
  15. Vonkeman H., Nugteren D. H., van Dorp D. A. The action of prostaglandin 15-hydroxydehydrogenase on various prostaglandins. Biochim Biophys Acta. 1969 Dec 17;187(4):581–583. doi: 10.1016/0005-2760(69)90058-7. [DOI] [PubMed] [Google Scholar]

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