Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1976 Apr;73(4):1145–1148. doi: 10.1073/pnas.73.4.1145

Isolation and structure of an untriakontapeptide with opiate activity from camel pituitary glands.

C H Li, D Chung
PMCID: PMC430217  PMID: 1063395

Abstract

The isolation of an untriakontapeptide from camel pituitary extracts has been described. Its structure has been determined and shown to be identical to the sequence of carboxyl-terminal 31 amino acids of ovine beta-lipotropin. The peptide possesses very low lipotropic activity but significant opiate activity.

Full text

PDF
1145

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chrétien M., Li C. H. Isolation, purification, and characterization of gamma-lipotropic hormone from sheep pituitary glands. Can J Biochem. 1967 Jul;45(7):1163–1174. doi: 10.1139/o67-133. [DOI] [PubMed] [Google Scholar]
  2. Cox B. M., Opheim K. E., Teschemacher H., Goldstein A. A peptide-like substance from pituitary that acts like morphine. 2. Purification and properties. Life Sci. 1975 Jun 15;16(12):1777–1782. doi: 10.1016/0024-3205(75)90272-6. [DOI] [PubMed] [Google Scholar]
  3. Cseh G., Barát E., Patthy A., Gráf L. Studies on the primary structure of human beta-lipotropic hormone. FEBS Lett. 1972 Apr 1;21(3):344–346. doi: 10.1016/0014-5793(72)80198-4. [DOI] [PubMed] [Google Scholar]
  4. Gráf L., Li C. H. Action of plasmin on ovine beta-lipotropin: revision of the carboxyl terminal sequence. Biochem Biophys Res Commun. 1973 Aug 21;53(4):1304–1309. doi: 10.1016/0006-291x(73)90607-4. [DOI] [PubMed] [Google Scholar]
  5. Hughes J., Smith T. W., Kosterlitz H. W., Fothergill L. A., Morgan B. A., Morris H. R. Identification of two related pentapeptides from the brain with potent opiate agonist activity. Nature. 1975 Dec 18;258(5536):577–580. doi: 10.1038/258577a0. [DOI] [PubMed] [Google Scholar]
  6. Lee V., Ramachandran J., Li C. H. Human pituitary growth hormone. Intrinsic lipolytic activity on rabbit fat cells. Arch Biochem Biophys. 1975 Aug;169(2):669–677. doi: 10.1016/0003-9861(75)90211-8. [DOI] [PubMed] [Google Scholar]
  7. Li C. H., Barnafi L., Chrétien M., Chung D. Isolation and amino-acid sequence of beta-LPH from sheep pituitary glands. Nature. 1965 Dec 11;208(5015):1093–1094. doi: 10.1038/2081093b0. [DOI] [PubMed] [Google Scholar]
  8. Li C. H., Danho W. O., Chung D., Rao A. J. Isolation, characterization, and amino acid sequence of melanotropins from camel pituitary glands. Biochemistry. 1975 Mar 11;14(5):947–952. doi: 10.1021/bi00676a011. [DOI] [PubMed] [Google Scholar]
  9. Li C. H., Dixon J. S., Lo T. B., Schmidt K. D., Pankov Y. A. Studies on pituitary lactogenic hormone. XXX. The primary structure of the sheep hormone. Arch Biochem Biophys. 1970 Dec;141(2):705–737. doi: 10.1016/0003-9861(70)90191-8. [DOI] [PubMed] [Google Scholar]
  10. Offord R. E. Electrophoretic mobilities of peptides on paper and their use in the determination of amide groups. Nature. 1966 Aug 6;211(5049):591–593. doi: 10.1038/211591a0. [DOI] [PubMed] [Google Scholar]
  11. Pankov Iu A. C-kontsevaia posledovatel'nost' aminokislotnykh ostatkov v molekule bych'ego beta-lipotropnogo gormona. Biokhimiia. 1972 Sep-Oct;37(5):1095–1096. [PubMed] [Google Scholar]
  12. Pankov Iu A., Iudaev N. A. Polnaia posledovatel'nost' aminokislotnykh ostatkov v molekule svinogo beta-lipotropina. Biokhimiia. 1972 Sep-Oct;37(5):991–1004. [PubMed] [Google Scholar]
  13. Sajgó C., Gráf L., Barát E., Sajgó M. Amino acid sequence of porcine beta-lipotropic hormone. Biochim Biophys Acta. 1971 Jan 19;229(1):276–278. doi: 10.1016/0005-2795(71)90344-8. [DOI] [PubMed] [Google Scholar]
  14. Simon E. J., Hiller J. M., Edelman I. Stereospecific binding of the potent narcotic analgesic (3H) Etorphine to rat-brain homogenate. Proc Natl Acad Sci U S A. 1973 Jul;70(7):1947–1949. doi: 10.1073/pnas.70.7.1947. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Woods K. R., Wang K. T. Separation of dansyl-amino acids by polyamide layer chromatography. Biochim Biophys Acta. 1967 Feb 21;133(2):369–370. doi: 10.1016/0005-2795(67)90078-5. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES