Fig. 6.

GSC loss induced by adult adipocyte-specific knockdown of amino acid transporters is independent of TOR signaling. (A) Ovaries at 10 days of adipocyte-specific CG12773 knockdown or Tsc1/Tsc2 induction showing retention of mature oocytes in subsets of ovarioles (‘blocked’ ovulation). Mature oocytes are recognizable by the presence of dorsal appendages (arrowheads). Scale bar: 500 μm. (B) Percentage of ovaries containing at least one ovariole that retains more than one mature oocyte at 10 days of adipocyte-specific amino acid transporter knockdown (left) or at different days of inhibition of TOR signaling (right). **P<0.01. Data from 0 d and 15 d time points are from one experiment, whereas 10 d represents three experiments. Number of ovaries analyzed is shown above each bar. (C) Average number of GSCs at different days of Gal80^ts; Lsp2-mediated induction of a dominant-negative RagA (RagA^T16N) or of Tsc1/Tsc2 transgenes showing that inhibition of TOR signaling has no effect on GSC maintenance. See supplementary material Fig. S6 for sample sizes and distribution. Control is GFP RNAi for A and B (left), and Gal80^ts; Lsp2 alone for B (right) and C.