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. 2014 Dec;141(23):4500–4512. doi: 10.1242/dev.113639

Fig. 2.

Fig. 2.

The ApjCreER lineage traces early SV sprouts. (A-F,H,I) Whole-mount confocal images immunostained for VE-cadherin (endothelium/endocardium) and cTnT (myocardium). Direct GFP fluorescence indicates lineage labeling. (A-C) Robust SV labeling at E10.5 (A) progressively spreads onto the heart at subsequent developmental stages (B,C). Little or no ventral labeling is detected at these stages. (D-F) High magnifications of the SV, endocardium (endo) and coronary vessels (CV) from the white-boxed regions in A-C. (G) Quantification of SV and coronary vessel labeling with a single E9.5 dose of tamoxifen. Error bars indicate one s.d. above and below the mean. Each dot represents the quantification for one heart. (H) Near complete labeling of the SV is achieved when tamoxifen is given once daily at E8.5, E9.5 and E10.5. (I) Depth-coded confocal images of red boxed regions in B and C showing that lineage-labeled cells first migrate subepicardially (red) at E11.5 and then into the myocardium (green) at E12.5. bi, blood island; cv, coronary vessels; la, left atrium; lv, left ventricle; ot, outflow tract; ra, right atrium; rv, right ventricle. Scale bars: 100 µm.