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. Author manuscript; available in PMC: 2015 Jan 22.
Published in final edited form as: Biochemistry. 2010 May 4;49(17):3602–3610. doi: 10.1021/bi9021999

Figure 5.

Figure 5

Effect of L499V mutation on PMA-dependent activation of α1 Na,K-ATPase mediated Rb+-transport. Na,K-ATPase-mediated transport was assayed in attached cells by measuring the ouabain-sensitive uptake of the K+ congener, 86Rb+. PKC activation was induced by a 5 min exposure of the cells to 10 μM PMA prior to the addition of Rb+, and compared to paired control plates of cells exposed for 5 min to the same amount of vehicle alone (DMSO). Values are means ± SEM (n = 8 to 11) of uptake induced by PMA exposure, expressed in percent of their paired controls (same transfection group, same passage, same day). Values were compared using one way ANOVA followed by Tukey's multiple comparison tests. NS, non significant. * P<0.05 vs wild-type α1.