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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1976 May;73(5):1432–1436. doi: 10.1073/pnas.73.5.1432

Comparative study of alkaline phosphatase activity in lymphocytes, mitogen-induced blasts, lymphoblastoid cell lines, acute myeloid leukemia, and chronic lymphatic leukemia cells.

H Neumann, E Klein, R Hauck-Granoth, S Yachnin, H Ben-Bassat
PMCID: PMC430310  PMID: 1064015

Abstract

Alkaline phosphatase [orthophosphoricmonoester phosphohydrolase (alkaline pH optimum), EC 3.1.3.1] purified from a Burkitt lymphoma cell line (Daudi) and Moloney-virus-induced murine leukemia (YAC) showed unique catalytic properties in substrate specificity and inhibition by cysteamine-S-phosphate. It migrated on polyacrylamide gel electrophoresis in a single activity band. Alkaline phosphatase with similar properties was found in several human lymphoblastoid cell lines, in chronic lymphatic leukemic cells, in organs of leukemic mice, and in sera of patients with certain lymphoproliferative disorders.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allan D., Crumpton M. J. Preparation and characterization of the plasma membrane of pig lymphocytes. Biochem J. 1970 Nov;120(1):133–143. doi: 10.1042/bj1200133. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Criss W. E. A review of isozymes in cancer. Cancer Res. 1971 Nov;31(11):1523–1542. [PubMed] [Google Scholar]
  3. Diehl V., Henle G., Henle W., Kohn G. Demonstration of a herpes group virus in cultures of peripheral leukocytes from patients with infectious mononucleosis. J Virol. 1968 Jul;2(7):663–669. doi: 10.1128/jvi.2.7.663-669.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. ELLMAN G. L. Tissue sulfhydryl groups. Arch Biochem Biophys. 1959 May;82(1):70–77. doi: 10.1016/0003-9861(59)90090-6. [DOI] [PubMed] [Google Scholar]
  5. FOLEY G. E., LAZARUS H., FARBER S., UZMAN B. G., BOONE B. A., MCCARTHY R. E. CONTINUOUS CULTURE OF HUMAN LYMPHOBLASTS FROM PERIPHERAL BLOOD OF A CHILD WITH ACUTE LEUKEMIA. Cancer. 1965 Apr;18:522–529. doi: 10.1002/1097-0142(196504)18:4<522::aid-cncr2820180418>3.0.co;2-j. [DOI] [PubMed] [Google Scholar]
  6. Greengard O. Enzymic differentiation in mammalian liver injection of fetal rats with hormones causes the premature formation of liver enzymes. Science. 1969 Feb 28;163(3870):891–895. doi: 10.1126/science.163.3870.891. [DOI] [PubMed] [Google Scholar]
  7. Haran-Ghera N., Hauch-Granoth R., Neumann H. Alkaline phosphatase activity in spontaneous and induced leukemias in SJL-J mice. Cancer Res. 1972 Nov;32(11):2475–2480. [PubMed] [Google Scholar]
  8. KLEIN E., KLEIN G. ANTIGENIC PROPERTIES OF LYMPHOMAS INDUCED BY THE MOLONEY AGENT. J Natl Cancer Inst. 1964 Mar;32:547–568. [PubMed] [Google Scholar]
  9. King E. J. The colorimetric determination of phosphorus. Biochem J. 1932;26(2):292–297. doi: 10.1042/bj0260292. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Klein E., Klein G., Nadkarni J. S., Nadkarni J. J., Wigzell H., Clifford P. Surface IgM-kappa specificity on a Burkitt lymphoma cell in vivo and in derived culture lines. Cancer Res. 1968 Jul;28(7):1300–1310. [PubMed] [Google Scholar]
  11. Klein E., Nilsson K., Yefenoff E. An established Burkitt's lymphoma line with cell membrane IgG. Clin Immunol Immunopathol. 1975 Mar;3(4):575–583. doi: 10.1016/0090-1229(75)90082-3. [DOI] [PubMed] [Google Scholar]
  12. Klein G., Dombos L., Gothoskar B. Sensitivity of Epstein-Barr virus (EBV) producer and non-producer human lymphoblastoid cell lines to superinfection with EB-virus. Int J Cancer. 1972 Jul 15;10(1):44–57. doi: 10.1002/ijc.2910100108. [DOI] [PubMed] [Google Scholar]
  13. Lozzio C. B., Lozzio B. B. Cytotoxicity of a factor isolated from human spleen. J Natl Cancer Inst. 1973 Feb;50(2):535–538. doi: 10.1093/jnci/50.2.535. [DOI] [PubMed] [Google Scholar]
  14. Minowada J., Onuma T., Moore G. E. Rosette-forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes. J Natl Cancer Inst. 1972 Sep;49(3):891–895. [PubMed] [Google Scholar]
  15. Neumann H., Boross L., Katchalski E. Hydrolysis of S-substituted monoesters of phosphorothioic acid by alkaline phosphatase from Escherichia coli. J Biol Chem. 1967 Jul 10;242(13):3142–3147. [PubMed] [Google Scholar]
  16. Neumann H., Moran E. M., Russell R. M., Rosenberg I. H. Distinct alkaline phosphatase in serum of patients with lymphatic leukemia and infectious mononucleosis. Science. 1974 Oct 11;186(4159):151–153. doi: 10.1126/science.186.4159.151. [DOI] [PubMed] [Google Scholar]
  17. Neumann H., Wilson K. J., Hauck-Granoth R., Haran-Ghera N. A comparative biochemical study of alkaline phosphatases in normal and leukemic mice. Cancer Res. 1971 Nov;31(11):1695–1701. [PubMed] [Google Scholar]
  18. Novogrodsky A., Katchalski E. Effect of phytohemagglutinin and prostaglandins on cyclic AMP synthesis in rat lymph node lymphocytes. Biochim Biophys Acta. 1970 Aug 14;215(2):291–296. doi: 10.1016/0304-4165(70)90027-9. [DOI] [PubMed] [Google Scholar]
  19. PETERSON E. A., CHIAZZE E. A. Some experimental factors in the gradient chromatography of serum proteins. Arch Biochem Biophys. 1962 Oct;99:136–147. doi: 10.1016/0003-9861(62)90256-4. [DOI] [PubMed] [Google Scholar]
  20. PULVERTAFT J. V. A STUDY OF MALIGNANT TUMOURS IN NIGERIA BY SHORT-TERM TISSUE CULTURE. J Clin Pathol. 1965 May;18:261–273. doi: 10.1136/jcp.18.3.261. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Smith I., Lightstone P. J., Perry J. D. Separation of human tissue alkaline phosphatases by electrophoresis on acrylamide disc gels. Clin Chim Acta. 1968 Mar;19(3):499–505. doi: 10.1016/0009-8981(68)90278-7. [DOI] [PubMed] [Google Scholar]
  22. Wilson K. J., Neumann H., Haran-Ghera N. Chronological appearance of alkaline phosphatase activity in virus-induced thymic lymphomas of C57BL-6 mice. Cancer Res. 1971 Nov;31(11):1702–1705. [PubMed] [Google Scholar]
  23. Yata J., Klein G. Some factors affecting membrane immunofluorescence reactivity of Burkitt lymphoma tissue culture cell lines. Int J Cancer. 1969 Nov 15;4(6):767–775. doi: 10.1002/ijc.2910040604. [DOI] [PubMed] [Google Scholar]
  24. de-Thé G., Ambrosioni J. C., Ho H. C., Kwan H. C. Lymphoblastoid transformation and presence of herpes-type viral particles in a Chinese nasopharyngeal tumour cultured in vitro. Nature. 1969 Feb 22;221(5182):770–771. doi: 10.1038/221770a0. [DOI] [PubMed] [Google Scholar]

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