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. 1976 May;73(5):1471–1475. doi: 10.1073/pnas.73.5.1471

Functional genetic expression of eukaryotic DNA in Escherichia coli.

K Struhl, J R Cameron, R W Davis
PMCID: PMC430318  PMID: 775490

Abstract

We have isolated a segment of DNA from the eukaryote Saccharomyces cerevisiae (baker's yeast) as a viable molecular hybrid of bacteriophage lambda DNA which, when integrated into the chromosome of an E. coli histidine auxotroph, allows this bacterium to grow in the absence of histidine. The nonrevertable, histidine auxotroph lacks the enzymatic activity of imidazole glycerol phosphate (IGP) dehydratase (EC 4.2.1.19). From genetic experiments, we conclude that expression of the segment of yeast DNA results in the production of a diffusible substance and that transcription necessary for the complementation is most likely initiated from the segment of eukaryotic DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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