Abstract
An assay for ribonuclease H (EC 3.1.4.34) is described which permits the estimation of the number and the type of cleavages produced by the enzyme when acting on the ribo moiety of a DNA-RNA hybrid substrate. With six different homopolymer hybrids tested, the number of enzymically induced breaks varied widely. The method is applicable to other endoribonucleases, phosphodiesterases, and phosphatases.
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Selected References
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- NEU H. C., HEPPEL L. A. NUCLEOTIDE SEQUENCE ANALYSIS OF POLYRIBONUCLEOTIDES BY MEANS OF PERIODATE OXIDATION FOLLOWED BY CLEAVAGE WITH AN AMINE. J Biol Chem. 1964 Sep;239:2927–2934. [PubMed] [Google Scholar]
- Stavrianopoulos J. G., Chargaff E. Purification and properties of ribonuclease H of calf thymus. Proc Natl Acad Sci U S A. 1973 Jul;70(7):1959–1963. doi: 10.1073/pnas.70.7.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stavrianopoulos J. G., Gambino-Giuffrida A., Chargaff E. Ribonuclease H of calf thymus: substrate specificity, activation, inhibition. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1087–1091. doi: 10.1073/pnas.73.4.1087. [DOI] [PMC free article] [PubMed] [Google Scholar]
- YU C. T., ZAMECNIK P. C. A hydrolytic procedure for ribonucleosides and its possible application to the sequential degradation of RNA. Biochim Biophys Acta. 1960 Dec 4;45:148–154. doi: 10.1016/0006-3002(60)91435-9. [DOI] [PubMed] [Google Scholar]