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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1976 May;73(5):1564–1567. doi: 10.1073/pnas.73.5.1564

In vitro transcription of chromatin in the presence of a mercurated nucleotide.

G F Crouse, J B Fodor, P Doty
PMCID: PMC430338  PMID: 1064024

Abstract

Mercurated uridine triphosphate has been used to label transcripts of chicken reticulocyte chromatin made with Escherichia coli RNA polymerase. The mercury-labeled RNA product can be completely separated from endogenous RNA sequences in the chromatin by passage through a sulfhydryl Sepharose column. Globin cDNA hybridization to the transcript shows that only 2.6 x 10-5 of the transcript is globin RNA. In contrast to this result, erythrocte chromatin transcript contains less than one tenth as many globin RNA sequences.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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