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. 2014 Dec 18;1(12):866–881. doi: 10.18632/oncoscience.110

Figure 7. Effects of cordycepin on EBV latent and lytic transcription.

Figure 7

Effects of cordycepin on EBV gene transcription were determined by performing an RT-qPCR assay using cDNA from SNU719 treated with cordycepin. (A) cDNA synthesized from RNA isolated from cordycepin-treated SNU719 cells was analyzed using an RT-qPCR assay. The relative transcription levels of EBV latent and lytic genes was determined. Compared to mock treatment, cordycepin treatment increases only the transcription of the EBV non-coding RNA EBER. However, most EBV genes were significantly downregulated by cordycepin. Cp, Wp, and Qp are EBV promoters activated depending on the EBV latency type [17]. (B) cDNA synthesized from RNA isolated from SNU719 cells cotreated with cordycepin, sodium butyrate, and TPA was analyzed using an RT-qPCR assay. The levels of EBV latent and lytic genes transcription significantly increased. P-values <0.05 (95% confidence) were considered statistically significant. CTCF BSs, binding sites where CTCF are known to bind in the KSHV genome [22]. P-values are listed at the bottom of the graph.