Neo-islets express islet hormones and transcription factors. Quantitative RT-PCR showing the expression of islet hormones (A) and transcription factors (B) involved in islet development at 8 weeks (n = 4–5). Data are mean ± SEM. *P ≤ 0.05. C and D: Representative sections of liver stained for Pdx-1 and Nkx 6.1 in various groups. Scale bar = 20 μm. 4w, 4 weeks; 8w, 8 weeks; 12w, 12 weeks; Ins-1, rat insulinoma cell line INS-1; Ins-2, rat insulinoma cell line INS-2; PP, pancreatic polypeptide; PV, portal vein; SST, somatostatin.