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. 1976 Jun;73(6):1984–1988. doi: 10.1073/pnas.73.6.1984

Transcriptional origin of Euglena chloroplast tRNAs*

Steven D Schwartzbach 1, Lanny I Hecker 1, W Edgar Barnett 1
PMCID: PMC430432  PMID: 16592326

Abstract

tRNA·DNA hybridization studies indicate that Euglena chloroplast tRNAs are transcriptional products of the chloroplast genome, which contains approximately 26 tRNA cistrons. Hybridization with purified chloroplast tRNAPhe and tRNAAsp shows that the chloroplast genome contains one cistron for each of these two species. No hybridization of chloroplast tRNA with nuclear DNA was observed. tRNAs from Euglena cytoplasm, Escherichia coli, and Agmenellum quadraduplicatum do not compete with chloroplast tRNA for hybridization with chloroplast DNA. Evidence is presented that photoinduction of chloroplast tRNAs is at the level of transcription rather than maturation of tRNA precursor molecules.

Keywords: hybridization, iodination, purified tRNAs, tRNA cistrons

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1984

Selected References

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