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. 1976 Jun;73(6):2033–2037. doi: 10.1073/pnas.73.6.2033

Stimulation of fetal hemoglobin synthesis in bone marrow cultures from adult individuals.

T H Papayannopoulou, M Brice, G Stamatoyannopoulos
PMCID: PMC430442  PMID: 1064874

Abstract

The regulation of fetal hemoglobin in adult erythroid cells was investigated with bone marrow cultures. Fetal hemoglobin (Hb F) was identified in individual erythroid colonies with fluorescent antibodies against Hb F and synthesis of gamma chains was determined with analyses of radioactive globins. The appearance of fetal hemoglobin in erythroid colonies was clonal. All the cells of the Hb F synthesizing colonies contained fetal hemoglobin. The frequency of erythroid colonies showing Hb F was higher than expected compared to the frequency of Hb F containing cells in the blood. Production of Hb F in culture, as shown by analysis of the radioactive globins, was 5 to 14 times higher than baseline Hb F synthesis. These results suggest that the ability for gamma chain synthesis in erythroid cells is determined at or above the level of the precursor cell from which the erythroid colonies, in vitro, derive (probably an erythropoietin responsive stem cell), and that stimulation of fetal hemoglobin synthesis in adult erythroid cells is possible.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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