Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1976 Jun;73(6):2082–2085. doi: 10.1073/pnas.73.6.2082

Biogenesis of mitochondria: molecular mapping of the mitochondrial genome of yeast.

A W Linnane, H B Lukins, P L Molloy, P Nagley, J Rytka, K S Sriprakash, M K Trembath
PMCID: PMC430453  PMID: 778855

Abstract

We have developed a new procedure for the detailed molecular mapping of any allele of the yeast (Saccharomyces cerevisiae) mitochondrial genome. The procedure employs a collection of different genetically characterized petite strains whose genomes have been physically defined by molecular hybridization. The map position of an allele is within the DNA segment common to all defined petites that can be shown by marker rescue to retain the locus. The same collection of petites can be used to locate the positions of mitochondrial rRNA and tRNA cistrons and DNA fragments produced by restriction endonucleases.

Full text

PDF
2082

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bell P. R. Are plastids autonomous? Symp Soc Exp Biol. 1970;24:109–127. [PubMed] [Google Scholar]
  2. Clark-Walker G. D., Miklos G. L. Complementation in cytoplasmic petite mutants of yeast to form respiratory competent cells. Proc Natl Acad Sci U S A. 1975 Jan;72(1):372–375. doi: 10.1073/pnas.72.1.372. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Faye G., Kujawa C., Fukuhara H. Physical and genetic organization of petite and grande yeast mitochondrial DNA. IV. In vivo transcription products of mitochondrial DNA and localization of 23 S ribosomal RNA in petite mutants of saccharomyces cerevisiae. J Mol Biol. 1974 Sep 5;88(1):185–203. doi: 10.1016/0022-2836(74)90304-0. [DOI] [PubMed] [Google Scholar]
  4. Howell N., Molloy P. L., Linnane A. W., Lukins H. B. Biogenesis of mitochondria 34. The synergistic interaction of nuclear and mitocohondrial mutations to produce resistance to high levels of mikamycin in Saccharomyces cerevisiae. Mol Gen Genet. 1974;128(1):43–54. doi: 10.1007/BF00267293. [DOI] [PubMed] [Google Scholar]
  5. Kleese R. A., Grotbeck R. C., Snyder J. R. Recombination among three mitochondrial genes in yeast (Saccharomyces cerevisiae). J Bacteriol. 1972 Nov;112(2):1023–1025. doi: 10.1128/jb.112.2.1023-1025.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kutzleb R., Schweyen R. J., Kaudewitz F. Extrachromosomal inheritance of paromomycin resistance in Saccharomyces cerevisiae. Genetic and biochemical characterization of mutants. Mol Gen Genet. 1973 Sep 5;125(1):91–98. doi: 10.1007/BF00292984. [DOI] [PubMed] [Google Scholar]
  7. Lancashire, Griffiths D. E. Studies on energy-linked reactions: genetic analysis of venturicidin-resistant mutants. Eur J Biochem. 1975 Feb 21;51(2):403–413. doi: 10.1111/j.1432-1033.1975.tb03940.x. [DOI] [PubMed] [Google Scholar]
  8. Molloy P. L., Howell N., Plummer D. T., Linnane A. W., Lukins H. B. Mitochondrial mutants of the yeast Saccharomyces cerevisiae showing resistance in vitro to chloramphenicol inhibition of mitochondrial protein synthesis. Biochem Biophys Res Commun. 1973 May 1;52(1):9–14. doi: 10.1016/0006-291x(73)90946-7. [DOI] [PubMed] [Google Scholar]
  9. Molloy P. L., Linnane A. W., Lukins H. B. Biogenesis of Mitochondria: Analysis of Deletion of Mitochondrial Antibiotic Resistance Markers in Petite Mutants of Saccharomyces cerevisiae. J Bacteriol. 1975 Apr;122(1):7–18. doi: 10.1128/jb.122.1.7-18.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Nagley P., Molloy P. L., Lukins H. B., Linnane A. W. Studies on mitochondrial gene purification using petite mutants of yeast: characterization of mutants enriched in ribosomal RNA cistrons. Biochem Biophys Res Commun. 1974 Mar 15;57(1):232–239. doi: 10.1016/s0006-291x(74)80381-5. [DOI] [PubMed] [Google Scholar]
  11. Sanders J. P., Heyting C., Borst P. The organization of genes in yeast mitochondrial DNA. I. The genes for large and small ribosomal RNA are far apart. Biochem Biophys Res Commun. 1975 Jul 22;65(2):699–707. doi: 10.1016/s0006-291x(75)80202-6. [DOI] [PubMed] [Google Scholar]
  12. Sriprakash K. S., Choo K. B., Nagley P., Linnane A. W. Physical mapping of mitochondria rRNA genes in Saccharomyces cerevisiae. Biochem Biophys Res Commun. 1976 Mar 8;69(1):85–91. doi: 10.1016/s0006-291x(76)80276-8. [DOI] [PubMed] [Google Scholar]
  13. Trembath M. K., Bunn C. L., Lukins H. B., Linnane A. W. Biogenesis of mitochondria 27. Genetic and biochemical characterisation of cytoplasmic and nuclear mutations to spiramycin resistance in Saccharomyces cerevisiae. Mol Gen Genet. 1973;121(1):35–48. doi: 10.1007/BF00353691. [DOI] [PubMed] [Google Scholar]
  14. Tzagoloff A., Akai A., Needleman R. B. Properties of cytoplasmic mutants of Saccharomyces cerevisiae with specific lesions in cytochrome oxidase. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2054–2057. doi: 10.1073/pnas.72.6.2054. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Wolf K., Dujon B., Slonimski P. P. Mitochondrial genetics. V. Multifactorial mitochondrial crosses involving a mutation conferring paromomycin-resistance in Saccharomyces cerevisiae. Mol Gen Genet. 1973 Sep 5;125(1):53–90. doi: 10.1007/BF00292983. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES