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. 1976 Jul;73(7):2191–2195. doi: 10.1073/pnas.73.7.2191

Synthesis of extensive, possibly complete, DNA copies of poliovirus RNA in high yields and at high specific activities.

D L Kacian, J C Myers
PMCID: PMC430492  PMID: 59925

Abstract

The synthesis of large, possibly complete, complementary DNA (cDNA) copies of poliovirus RNA by avian myeloblastosis virus DNA polymerase is described. The cDNA consists of two size classes, the larger of which is approximately 7500 nucleotides. In the presence of excess deoxynucleoside triphosphates, ribonucleoside triphosphates, or sodium pyrophosphate, only the larger material is obtained. Yields of the large cDNA are 50-75% of the input RNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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