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. 1976 Jul;73(7):2266–2269. doi: 10.1073/pnas.73.7.2266

Deposition of histone onto the replicating chromosome: newly synthesized histone is not found near the replication fork.

V Jackson, D Granner, R Chalkley
PMCID: PMC430523  PMID: 1065875

Abstract

We have studied the site of deposition of newly synthesized histone. It appears to be randomly distributed over the chromosomal material and does not become associated specifically with immediately post-replicational DNA, nor is it deposited in discrete continuous regions distal to the sites of DNA synthesis. The newly synthesized DNA, however, rapidly acquires a complement of chromosomal proteins; presumably, preexisting histones must migrate to become associated with post-replicational DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Borun T. W., Scharff M. D., Robbins E. Rapidly labeled, polyribosome-associated RNA having the properties of histone messenger. Proc Natl Acad Sci U S A. 1967 Nov;58(5):1977–1983. doi: 10.1073/pnas.58.5.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Breindl M., Gallwitz D. On the translational control of histone synthesis. Quantitation of biologically active histone mRNA from synchronized HeLa cells and its translation in different cell-free systems. Eur J Biochem. 1974 Jun 1;45(1):91–97. doi: 10.1111/j.1432-1033.1974.tb03533.x. [DOI] [PubMed] [Google Scholar]
  3. Comings D. E., Kakefuda T. Initiation of deoxyribonucleic acid replication at the nuclear membrane in human cells. J Mol Biol. 1968 Apr 14;33(1):225–229. doi: 10.1016/0022-2836(68)90290-8. [DOI] [PubMed] [Google Scholar]
  4. Comings D. E. The structure and function of chromatin. Adv Hum Genet. 1972;3:237–431. doi: 10.1007/978-1-4757-4429-3_5. [DOI] [PubMed] [Google Scholar]
  5. Fakan S., Turner G. N., Pagano J. S., Hancock R. Sites of replication of chromosomal DNA in a eukaryotic cell. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2300–2305. doi: 10.1073/pnas.69.8.2300. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gallwitz D., Mueller G. C. Histone synthesis in vitro on HeLa cell microsomes. The nature of the coupling to deoxyribonucleic acid synthesis. J Biol Chem. 1969 Nov 10;244(21):5947–5952. [PubMed] [Google Scholar]
  7. Hancock R. Interphase chromosomal deoxyribonucleoprotein isolated as a discrete structure from cultured cells. J Mol Biol. 1974 Jul 5;86(3):649–663. doi: 10.1016/0022-2836(74)90187-9. [DOI] [PubMed] [Google Scholar]
  8. Huberman J. A., Tsai A., Deich R. A. DNA replication sites within nuclei of mammalian cells. Nature. 1973 Jan 5;241(5384):32–36. doi: 10.1038/241032a0. [DOI] [PubMed] [Google Scholar]
  9. Jackson V., Chalkley R. Separation of newly synthesized nucleohistone by equilibrium centrifugation in cesium chloride. Biochemistry. 1974 Sep 10;13(19):3952–3956. doi: 10.1021/bi00716a021. [DOI] [PubMed] [Google Scholar]
  10. Jackson V., Granner D. K., Chalkley R. Deposition of histones onto replicating chromosomes. Proc Natl Acad Sci U S A. 1975 Nov;72(11):4440–4444. doi: 10.1073/pnas.72.11.4440. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ling V., Trevithick J. R., Dixon G. H. The biosynthesis of protamine in trout testis. I. Intracellular site of synthesis. Can J Biochem. 1969 Jan;47(1):51–60. doi: 10.1139/o69-009. [DOI] [PubMed] [Google Scholar]
  12. Oliver D., Granner D., Chalkley R. Identification of a distinction between cytoplasmic histone synthesis and subsequent histone deposition within the nucleus. Biochemistry. 1974 Feb 12;13(4):746–749. doi: 10.1021/bi00701a017. [DOI] [PubMed] [Google Scholar]
  13. Robbins E., Borun T. W. The cytoplasmic synthesis of histones in hela cells and its temporal relationship to DNA replication. Proc Natl Acad Sci U S A. 1967 Feb;57(2):409–416. doi: 10.1073/pnas.57.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ruiz-Carrillo A., Wangh L. J., Allfrey V. G. Processing of newly synthesized histone molecules. Science. 1975 Oct 10;190(4210):117–128. doi: 10.1126/science.1166303. [DOI] [PubMed] [Google Scholar]
  15. Seale R. L., Simpson R. T. Effects of cycloheximide on chromatin biosynthesis. J Mol Biol. 1975 May 25;94(3):479–501. doi: 10.1016/0022-2836(75)90216-8. [DOI] [PubMed] [Google Scholar]
  16. Seale R. L. Temporal relationships of chromatin protein synthesis, DNA synthesis, and assembly of deoxyribonucleoprotein. Proc Natl Acad Sci U S A. 1976 Jul;73(7):2270–2274. doi: 10.1073/pnas.73.7.2270. [DOI] [PMC free article] [PubMed] [Google Scholar]

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