Abstract
Bovine adrenal cortical tissue contains a lipoidal derivative of pregnenolone (3beta-hydroxy-pregn-5-en-20-one) from which the free steroid can be liberated by treatment with alkali. Evidence for the presence of such an entity comes from examination of a nonpolar extract of tissue from which pregnenolone and its sulfate had been removed by chromatography. Treatment of the nonpolar fraction with alkali followed by exhaustive chromatographic analysis led to the detection of pregnenolone. The steroid was identified by both gas chromatography/mass spectrometry and double isotope procedures. Quantitative analysis indicated that the three forms of pregnenolone are present in bovine adrenal cortical tissue in the following amounts (mug/kg): lipoidal derivative, 290; free steroid, 435; and sulfate, 65. Because the only known metabolic function of pregnenolone is to serve as a precursor of the steroid hormones, these findings have far-reaching implications for steroid hormone biochemistry.
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Selected References
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- HECHTER O. Concerning possible mechanisms of hormone action. Vitam Horm. 1955;(13):293–346. doi: 10.1016/s0083-6729(08)61029-8. [DOI] [PubMed] [Google Scholar]
- Hochberg R. B., Hoeven TA vander, Welch M., Lieberman S. A simple and precise assay of the enzymatic conversion of cholesterol into pregnenolone. Biochemistry. 1974 Jan 29;13(3):603–609. doi: 10.1021/bi00700a031. [DOI] [PubMed] [Google Scholar]
- Hochberg R. B., McDonald P. D., Feldman M., Lieberman S. Fragments formed by the side chain cleavage of a 20-aryl analog of 20alpha-hydroxycholesterol by adrenal mitochondria. J Biol Chem. 1976 Apr 10;251(7):2087–2093. [PubMed] [Google Scholar]
- Hochberg R. B., McDonald P. D., Ponticorvo L., Lieberman S. Identification of 17-methyl-18-norandrosta-5,13(17-dien-3beta-ol, the C19 fragment formed by adrenal side chain cleavage of a 20-aryl analog of (20S)-20-hydroxycholesterol. J Biol Chem. 1976 Dec 10;251(23):7336–7342. [PubMed] [Google Scholar]
- LIPSETT M. B., COFFMAN G. D., NIXON W. E. LACK OF ACTIVITY OF DEHYDROEPIANDROSTERONE SULFATE IN METABOLIC BALANCE STUDIES. J Clin Endocrinol Metab. 1965 Jul;25:993–994. doi: 10.1210/jcem-25-7-993. [DOI] [PubMed] [Google Scholar]
- Roberts K. D., Bandy L., Lieberman S. The occurrence and metabolism of 20 alpha-hydroxycholesterol in bovine adrenal preparations. Biochemistry. 1969 Mar;8(3):1259–1270. doi: 10.1021/bi00831a061. [DOI] [PubMed] [Google Scholar]