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. 1976 Jul;73(7):2304–2307. doi: 10.1073/pnas.73.7.2304

Dietary and hormonal regulation of the content of acetyl coenzyme A carboxylase-synthesizing polysomes in rat liver.

S Nakanishi, T Tanabe, S Horikawa, S Numa
PMCID: PMC430540  PMID: 7784

Abstract

Specific polysomes involved the the synthesis of acetyl-CoA carboxylase [acetyl-CoA: carbon-dioxide ligase (ADP-forming), EC 6.4.1.2] have been identified by the binding of 125I-labeled antiacetyl-CoA carboxylase to rat liver polysomes. The binding is highly specific and occurs through the recognition of the nascent peptide chains on polysomes. With the use of the 125I-labeled antibody binding technique, it has been demonstrated that the relative content of acetyl-CoA carboxylase-synthesizing polysomes in the liver correlates well with the rate of hepatic synthesis of the enzyme in rats subjected to different dietary conditions as well as in alloxan-diabetic rats with or without insulin treatment.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALLMANN D. W., HUBBARD D. D., GIBSON D. M. FATTY ACID SYNTHESIS DURING FAT-FREE REFEEDING OF STARVED RATS. J Lipid Res. 1965 Jan;6:63–74. [PubMed] [Google Scholar]
  2. Alberts A. W., Strauss A. W., Hennessy S., Vagelos P. R. Regulation of synthesis of hepatic fatty acid synthetase: binding of fatty acid synthetase antibodies to polysomes. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3956–3960. doi: 10.1073/pnas.72.10.3956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hashimoto T., Numa S. Kinetic studies on the reaction mechanism and the citrate activation of liver acetyl coenzyme A carboxylase. Eur J Biochem. 1971 Feb 1;18(3):319–331. doi: 10.1111/j.1432-1033.1971.tb01247.x. [DOI] [PubMed] [Google Scholar]
  4. Kitajima K., Tashiro S., Numa S. Acetyl-coenzyme-A carboxylase in cultured hepatocytes. Effects of exogenous fatty acids on the content, synthesis and degradation of the enzyme. Eur J Biochem. 1975 Jun;54(2):373–383. doi: 10.1111/j.1432-1033.1975.tb04148.x. [DOI] [PubMed] [Google Scholar]
  5. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  6. Majerus P. W., Kilburn E. Acetyl coenzyme A carboxylase. The roles of synthesis and degradation in regulation of enzyme levels in rat liver. J Biol Chem. 1969 Nov 25;244(22):6254–6262. [PubMed] [Google Scholar]
  7. NUMA S., MATSUHASHI M., LYNEN F. [On disorders of fatty acid synthesis in hunger and alloxan diabetes. I. Fatty acid synthesis in the liver of normal and fasting rats]. Biochem Z. 1961;334:203–217. [PubMed] [Google Scholar]
  8. Nakanishi S., Numa S. Purification of rat liver acetyl coenzyme A carboxylase and immunochemical studies on its synthesis and degradation. Eur J Biochem. 1970 Sep;16(1):161–173. doi: 10.1111/j.1432-1033.1970.tb01068.x. [DOI] [PubMed] [Google Scholar]
  9. Numa S., Yamashita S. Regulation of lipogenesis in animal tissues. Curr Top Cell Regul. 1974;8(0):197–246. doi: 10.1016/b978-0-12-152808-9.50012-2. [DOI] [PubMed] [Google Scholar]
  10. Palacios R., Palmiter R. D., Schimke R. T. Identification and isolation of ovalbumin-synthesizing polysomes. I. Specific binding of 125 I-anti-ovalbumin to polysomes. J Biol Chem. 1972 Apr 25;247(8):2316–2321. [PubMed] [Google Scholar]
  11. Palmiter R. D., Palacios R., Schimke R. T. Identification and isolation of ovalbumin-synthesizing polysomes. II. Quantification and immunoprecipitation of polysomes. J Biol Chem. 1972 May 25;247(10):3296–3304. [PubMed] [Google Scholar]
  12. Taylor J. M., Schimke R. T. Specific binding of albumin antibody to rat liver polysomes. J Biol Chem. 1974 Jun 10;249(11):3597–3601. [PubMed] [Google Scholar]
  13. Taylor J. M., Schimke R. T. Synthesis of rat liver albumin in a rabbit reticulocyte cell-free protein-synthesizing system. J Biol Chem. 1973 Nov 25;248(22):7661–7668. [PubMed] [Google Scholar]
  14. WIELAND O., NEUFELDT I., NUMA S., LYNEN F. [On disorders in fatty acid synthesis in hunger and alloxan diabetes. II. Fatty acid synthesis in the liver of alloxan diabetic rats]. Biochem Z. 1963;336:455–459. [PubMed] [Google Scholar]

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