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. 1976 Jul;73(7):2356–2360. doi: 10.1073/pnas.73.7.2356

Host restriction of Friend leukemia virus: synthesis and integration of the provirus.

M M Sveda, R Soeiro
PMCID: PMC430564  PMID: 1065886

Abstract

Host restriction of exogenous infection by murine leukemia viruses is controlled in vitro predominantly by the murine Fv-1 locus. The mechanism of this host restriction was investigated by comparing the early events in the replication of N-tropic versus B-tropic Friend leukemia virus in NIH 3T3 cells. These cells, which are Fv-1nn in type, are permissive for the N-tropic strain, but nonpermissive for the B-tropic strain, which replicates permissively in Balb/c cells. We have studied the synthesis, intracellular location, and molecular form of virus-specific DNA early in replication by means of molecular hybridization with a virus-specific DNA probe. Our results suggest that in the permissive infection viral DNA rapidly becomes integrated with cellular DNA. However, in the nonpermissive infection, although almost equal amounts of both positive and negative strand viral DNA are synthesized, integration of the provirus does not occur.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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