Abstract
Anionic glycopeptides and glycosaminoglycans synthesized by embryonic neural tube (epithelium) and neural crest (mesenchymal outgrowth) developing in vitro were examined. The profile of surface glycopeptides is relatively simple (two major ones for neural crest; four major ones for neural tube). There is one major glycopeptide found in the medium which is present only in trace amounts on the cells. Both hyaluronate and chondroitin sulfate are synthesized. Hyaluronate is predominantly cell associated; chondroitin sulfate is found predominantly in the medium. It is suggested that independent sorting of these relatively few glycopeptides can result in qualitative surface differences. Morphological state (epithelial or mesenchymal) and differentiated state may be related to these differences as well as to quantitative differences in surface glycosaminoglycans.
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- Brown J. C. Cell surface glycoproteins. I. Accumulation of a glycoprotein on the outer surface of mouse LS cells during mitosis. J Supramol Struct. 1972;1(1):1–7. doi: 10.1002/jss.400010102. [DOI] [PubMed] [Google Scholar]
- Buck C. A., Glick M. C., Warren L. A comparative study of glycoproteins from the surface of control and Rous sarcoma virus transformed hamster cells. Biochemistry. 1970 Nov 10;9(23):4567–4576. doi: 10.1021/bi00825a016. [DOI] [PubMed] [Google Scholar]
- Buck C. A., Glick M. C., Warren L. Effect of growth on the glycoproteins from the surface of control and Rous sarcoma virus transformed hamster cells. Biochemistry. 1971 May 25;10(11):2176–2180. doi: 10.1021/bi00787a034. [DOI] [PubMed] [Google Scholar]
- Cohen A. M., Konigsberg I. R. A clonal approach to the problem of neural crest determination. Dev Biol. 1975 Oct;46(2):262–280. doi: 10.1016/0012-1606(75)90104-9. [DOI] [PubMed] [Google Scholar]
- Manasek F. J. Glycoprotein synthesis and tissue interactions during establishment of the functional embryonic chick heart. J Mol Cell Cardiol. 1976 May;8(5):389–402. doi: 10.1016/0022-2828(76)90043-2. [DOI] [PubMed] [Google Scholar]
- Meezan E., Wu H. C., Black P. H., Robbins P. W. Comparative studies on the carbohydrate-containing membrane components of normal and virus-transformed mouse fibroblasts. II. Separation of glycoproteins and glycopeptides by sephadex chromatography. Biochemistry. 1969 Jun;8(6):2518–2524. doi: 10.1021/bi00834a039. [DOI] [PubMed] [Google Scholar]
- Pratt R. M., Larsen M. A., Johnston M. C. Migration of cranial neural crest cells in a cell-free hyaluronate-rich matrix. Dev Biol. 1975 Jun;44(2):298–305. doi: 10.1016/0012-1606(75)90400-5. [DOI] [PubMed] [Google Scholar]