Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1976 Jul;73(7):2419–2423. doi: 10.1073/pnas.73.7.2419

Deficiency of gamma-ray excision repair in skin fibroblasts from patients with Fanconi's anemia.

J F Remsen, P A Cerutti
PMCID: PMC430591  PMID: 1065896

Abstract

The capacity of preparations of skin fibroblasts from normal individuals and patients with Fanconi's anemia to excise gamma-ray products of the 5,6-dihydroxydihydrothymine type from exogenous DNA was investigated. The excision capacity of whole-cell homogenates of fibroblasts from two of four patients with Fanconi's anemia was substantially below normal. This repair deficiency was further pronounced in nuclear preparations from cells of the same two patients.

Full text

PDF
2419

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bacchetti S., Benne R. Purification and characterization of an endonuclease from calf thymus acting on irradiated DNA. Biochim Biophys Acta. 1975 May 16;390(3):285–297. doi: 10.1016/0005-2787(75)90349-4. [DOI] [PubMed] [Google Scholar]
  2. Duker N. J., Teebor G. W. Different ultraviolet DNA endonuclease activity in human cells. Nature. 1975 May 1;255(5503):82–84. doi: 10.1038/255082a0. [DOI] [PubMed] [Google Scholar]
  3. Dunlap B., Cerutti P. Apyrimidinic sites in gamma-irradiated DNA. FEBS Lett. 1975 Mar 1;51(1):188–190. doi: 10.1016/0014-5793(75)80884-2. [DOI] [PubMed] [Google Scholar]
  4. Fujiwara Y., Tatsumi M. Repair of mitomycin C damage to DNA in mammalian cells and its impairment in Fanconi's anemia cells. Biochem Biophys Res Commun. 1975 Sep 16;66(2):592–598. doi: 10.1016/0006-291x(75)90551-3. [DOI] [PubMed] [Google Scholar]
  5. Hariharan P. V., Cerutti P. A. Excision of damaged thymine residues from gamma-irradiated poly(dA-dT) by crude extracts of Escherichia coli. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3532–3536. doi: 10.1073/pnas.71.9.3532. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Higurashi M., Conen P. E. In vitro chromosomal radiosensitivity in Fanconi's anemia. Blood. 1971 Sep;38(3):336–342. [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. Latt S. A., Stetten G., Juergens L. A., Buchanan G. R., Gerald P. S. Induction by alkylating agents of sister chromatid exchanges and chromatid breaks in Fanconi's anemia. Proc Natl Acad Sci U S A. 1975 Oct;72(10):4066–4070. doi: 10.1073/pnas.72.10.4066. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Levine E. M. Mycoplasma contamination of animal cell cultures: a simple, rapid detection method. Exp Cell Res. 1972 Sep;74(1):99–109. doi: 10.1016/0014-4827(72)90484-3. [DOI] [PubMed] [Google Scholar]
  10. Mattern M. R., Cerutti P. A. Age-dependent excision repair of damaged thymine from gamma-irradiated DNA by isolated nuclei from human fibroblasts. Nature. 1975 Apr 3;254(5499):450–452. doi: 10.1038/254450a0. [DOI] [PubMed] [Google Scholar]
  11. McMacken R., Kessler S., Boyce R. Strand breakage of coliphage lambda DNA supercoils in infected lysogens. I. Genetic and biochemical evidence for two types of nicking processes. Virology. 1975 Aug;66(2):356–371. doi: 10.1016/0042-6822(75)90209-3. [DOI] [PubMed] [Google Scholar]
  12. Poon P. K., O'Brien R. L., Parker J. W. Defective DNA repair in Fanconi's anaemia. Nature. 1974 Jul 19;250(463):223–225. doi: 10.1038/250223a0. [DOI] [PubMed] [Google Scholar]
  13. Sasaki M. S. Is Fanconi's anaemia defective in a process essential to the repair of DNA cross links? Nature. 1975 Oct 9;257(5526):501–503. doi: 10.1038/257501a0. [DOI] [PubMed] [Google Scholar]
  14. Sasaki M. S., Tonomura A. A high susceptibility of Fanconi's anemia to chromosome breakage by DNA cross-linking agents. Cancer Res. 1973 Aug;33(8):1829–1836. [PubMed] [Google Scholar]
  15. Swinehart J. L., Lin W. S., Cerutti P. A. Gamma-ray induced damage in thymine in mononucleotide mixtures, and in single- and double-stranded DNA. Radiat Res. 1974 May;58(2):166–175. [PubMed] [Google Scholar]
  16. Teebor G. W., Duker N. J. Human endonuclease activity for DNA apurinic sites. Nature. 1975 Dec 11;258(5535):544–547. doi: 10.1038/258544a0. [DOI] [PubMed] [Google Scholar]
  17. Verly W. G., Paquette Y., Thibodeau L. Nuclease for DNA apurinic sites may be involved in the maintenance of DNA in normal cells. Nat New Biol. 1973 Jul 18;244(133):67–69. doi: 10.1038/newbio244067a0. [DOI] [PubMed] [Google Scholar]
  18. Yamamoto K. R., Alberts B. M., Benzinger R., Lawhorne L., Treiber G. Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification. Virology. 1970 Mar;40(3):734–744. doi: 10.1016/0042-6822(70)90218-7. [DOI] [PubMed] [Google Scholar]
  19. van Lancker J. L., Tomura T. Purification and some properties of a mammalian repair endonuclease. Biochim Biophys Acta. 1974 Jun 14;353(1):99–114. doi: 10.1016/0005-2787(74)90101-4. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES