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. 1977 Mar;74(3):1185–1189. doi: 10.1073/pnas.74.3.1185

Genetic control of renin activity in the submaxillary gland of the mouse.

C M Wilson, E G Erdös, J F Dunn, J D Wilson
PMCID: PMC430645  PMID: 15253

Abstract

Administration of androgen to female mice is known to increase the level of several proteins in the submaxillary gland, including nerve growth factor, epidermal growth factor, esteroproteolytic activity, and renin. In the present study, renin activity has been assessed in extracts of submaxillary gland of female mice from two inbred strains (SWR/J and C57BL/10J), from F1 and F2 hybrids, and from backcrosses between F1 and parental strains. In both uninduced and induced mice, renin activity of submaxillary gland was more than 100-fold greater in SWR/J than in C57BL/10J mice as measured by either an enzymatic assay or an immunodiffusion method. This difference was not due to differences in plasma testosterone levels between the strains, and the enzymes from the two strains had similar pH optima, substrate specificities, heat stabilities, and apparent Michaelis constants. In the submaxillary gland the difference was relatively specific for renin because increases in esteroproteolytic activity, nerve growth factor, and epidermal growth factor after androgen treatment appeared to be similar in both strains. Studies with the various hybrids indicate that the difference in renin activity between the two strains is apparently due to a single regulatory gene.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Angeletti R. A., Angeletti P. U., Calissano P. Testosterone induction of estero-proteolytic activity in the mouse submaxillary gland. Biochim Biophys Acta. 1967 Jul 11;139(2):372–381. doi: 10.1016/0005-2744(67)90041-1. [DOI] [PubMed] [Google Scholar]
  2. BING J. LOCATION OF RENIN (OR A RENIN-LIKE SUBSTANCE) IN THE SUBMAXILLARY GLANDS OF ALBINO MICE. Acta Pathol Microbiol Scand. 1965;64:203–212. doi: 10.1111/apm.1965.64.2.203. [DOI] [PubMed] [Google Scholar]
  3. Byyny R. L., Orth D. N., Cohen S., Doyne E. S. Epidermal growth factor: effects of androgens and adrenergic agents. Endocrinology. 1974 Sep;95(3):776–782. doi: 10.1210/endo-95-3-776. [DOI] [PubMed] [Google Scholar]
  4. Calissano P., Angeletti P. U. Testosterone effect on the synthetic rate of two esteropeptidases in the mouse submaxillary gland. Biochim Biophys Acta. 1968 Feb 1;156(1):51–58. doi: 10.1016/0304-4165(68)90103-7. [DOI] [PubMed] [Google Scholar]
  5. Chiang T. S., Erdös E. G., Miwa I., Tague L. L., Coalson J. J. Isolation from a salivary gland of granules containing renin and kallikrein. Circ Res. 1968 Oct;23(4):507–517. doi: 10.1161/01.res.23.4.507. [DOI] [PubMed] [Google Scholar]
  6. Cohen S., Taylor J. M., Murakami K., Michelakis A. M., Inagami T. Isolation and characterization of renin-like enzymes from mouse submaxillary glands. Biochemistry. 1972 Nov 7;11(23):4286–4293. doi: 10.1021/bi00773a015. [DOI] [PubMed] [Google Scholar]
  7. Dunn J. F., Wilson J. D. Developmental study of androgen responsiveness in the submandibular gland of the mouse. Endocrinology. 1975 Jun;96(6):1571–1578. doi: 10.1210/endo-96-6-1571. [DOI] [PubMed] [Google Scholar]
  8. Gecse A., Wilson C. M., Erdös E. G. Induction of particle-bound renin and arginine esterase by testosterone in the mouse. Biochem Pharmacol. 1976 Apr 1;25(7):763–768. doi: 10.1016/0006-2952(76)90143-x. [DOI] [PubMed] [Google Scholar]
  9. Ishii D. N., Shooter E. M. Regulation of nerve growth factor synthesis in mouse submaxillary glands by testosterone. J Neurochem. 1975 Dec;25(6):843–851. doi: 10.1111/j.1471-4159.1975.tb04416.x. [DOI] [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. Michelakis A. M., Cohen S., Taylor J., Murakami K., Inagami T. Studies on the characterization of pure submaxillary gland renin. Proc Soc Exp Biol Med. 1974 Oct;147(1):118–121. doi: 10.3181/00379727-147-38293. [DOI] [PubMed] [Google Scholar]
  12. Michelakis A. M., Yoshida H., Menzie J., Murakami K., Inagami T. A radioimmunoassay for the direct measurement of renin in mice and its application to submaxillary gland and kidney studies. Endocrinology. 1974 Apr;94(4):1101–1105. doi: 10.1210/endo-94-4-1101. [DOI] [PubMed] [Google Scholar]
  13. Milewich L., Gomez-Sanchez C., MacDonald P., Siiteri P. K. Radioimmunoassay of androstenedione: the steroid molecule as a probe for antibody specificity. J Steroid Biochem. 1975 Oct;6(10):1381–1387. doi: 10.1016/0022-4731(75)90073-4. [DOI] [PubMed] [Google Scholar]
  14. OUCHTERLONY O. Diffusion-in-gel methods for immunological analysis. Prog Allergy. 1958;5:1–78. [PubMed] [Google Scholar]
  15. Oliver W. J., Gross F. Effect of testosterone and duct ligation on submaxillary renin-like principle. Am J Physiol. 1967 Aug;213(2):341–346. doi: 10.1152/ajplegacy.1967.213.2.341. [DOI] [PubMed] [Google Scholar]
  16. Schenkein I., Levy M., Bueker E. D., Wilson J. D. Immunological and enzymatic evidence for the absence of an esteroproteolytic enzyme (protease "D") in the submandibular gland of the Tfm mouse. Endocrinology. 1974 Mar;94(3):840–844. doi: 10.1210/endo-94-3-840. [DOI] [PubMed] [Google Scholar]
  17. Skeggs L. T., Lentz K. E., Kahn J. R., Dorer F. E., Levine M. Pseudorenin. A new angiotensin-forming enzyme. Circ Res. 1969 Oct;25(4):451–462. doi: 10.1161/01.res.25.4.451. [DOI] [PubMed] [Google Scholar]
  18. Swank R. T., Paigen K., Ganschow R. E. Genetic control of glucuronidase induction in mice. J Mol Biol. 1973 Dec 5;81(2):225–243. doi: 10.1016/0022-2836(73)90191-5. [DOI] [PubMed] [Google Scholar]
  19. TURRIAN H. [Some characteristics of the hypertensive factor contained in the salivary glands of mice]. Helv Physiol Pharmacol Acta. 1960;18:259–265. [PubMed] [Google Scholar]
  20. Trautschold I., Werle E., Schmal A., Hendrikoff N. G. Die hormonelle Beeinflussung des Isrenin-Spiegels der Submandibularisdrüse der weissen Maus und zur Lokalisierung des Enzyms in der Drüse. Hoppe Seylers Z Physiol Chem. 1966;344(4):232–243. [PubMed] [Google Scholar]
  21. WERLE E., TRAUTSCHOLD I., SCHMAL A. UBER EIN ISO-ENZYM DES RENINS UND UEBER DIE ISOLIERUNG EINES BIOLOGISCH AKTIVEN SPALTPRODUKTES SEINES SUBSTRATES. Hoppe Seylers Z Physiol Chem. 1963;332:79–87. doi: 10.1515/bchm2.1963.332.1.79. [DOI] [PubMed] [Google Scholar]
  22. WERLE E., VOGEL R., GOLDEL L. F. Uber ein blutdrucksteigerndes Prinzip in Extrakten aus der Glandula submaxillaris der weissen Maus. Naunyn Schmiedebergs Arch Exp Pathol Pharmakol. 1957;230(3):236–244. [PubMed] [Google Scholar]
  23. de Jong W., Lovenberg W., Sjoerdsma A. Renin-like activity in submaxillary gland in several strains of rats including the spontaneously hypertensive rat. Biochem Pharmacol. 1972 Aug 1;21(15):2123–2129. doi: 10.1016/0006-2952(72)90165-7. [DOI] [PubMed] [Google Scholar]

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