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CWD Isolate Titration in Elk21- Cells. Elk21- cells were exposed to serial dilutions of two different CWD isolates (elk, 132M, and white-tailed deer, 95Q/96G) and the generation of PrPSc determined by ELISPOT analysis following three passages of the cells. Elk21- cells demonstrated a dose-dependent PrPSc spot response to elk and white-tailed deer CWD. N = 6, mean ± SEM.
