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. 1976 Aug;73(8):2559–2563. doi: 10.1073/pnas.73.8.2559

Guanosine 3':5'-cyclic monophosphate binding proteins in rat tissues.

T M Lincoln, C L Hall, C R Park, J D Corbin
PMCID: PMC430687  PMID: 8775

Abstract

Rat tissues were surveyed for proteins which bind cGMP. Binding activity was high in extracts of lung, cerebellum, and small intestine, but was low in those of liver, adipose tissue, and skeletal muscle. DEAE-cellulose chromatography resolved two peaks of cGMP-binding activity in most tissues. The binding protein in peak 1 was eluted in the flow-through volume and was most abundant in extracts of intestine. It had a sedimentation coefficient of 6S and was highly specific for cGMP at pH 7.0 (dissociation constant KD=0.05 muM). No cGMP-dependent histone kinase activity was found for this peak. The binding protein in peak 2 was eluted by 0.05-0.15 M NaCl and was the predominant binding substance in lung, cerebellum, and heart. It had a sedimentation coefficient of 8S and binding was also highly specific for cGMP, with a KD of 0.05 muM. This peak of binding activity was associated with cGMP-dependent protein kinase activity which could be purified approximately 200-fold by Sepharose 6B chromatography. Cyclic GMP dependency of kinase activity was observed only at low histone concentrations. The abundance of one or both the above binding proteins correlated with the known basal levels of cGMP in the tissues.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chambers D. A., Martin D. W., Jr, Weinstein Y. The effect of cyclic nucleotides on purine biosynthesis and the induction of PRPP synthetase during lymphocyte activation. Cell. 1974 Dec;3(4):375–380. doi: 10.1016/0092-8674(74)90053-1. [DOI] [PubMed] [Google Scholar]
  2. Corbin J. D., Keely S. L., Park C. R. The distribution and dissociation of cyclic adenosine 3':5'-monophosphate-dependent protein kinases in adipose, cardiac, and other tissues. J Biol Chem. 1975 Jan 10;250(1):218–225. [PubMed] [Google Scholar]
  3. Corbin J. D., Soderling T. R., Park C. R. Regulation of adenosine 3',5'-monophosphate-dependent protein kinase. I. Preliminary characterization of the adipose tissue enzyme in crude extracts. J Biol Chem. 1973 Mar 10;248(5):1813–1821. [PubMed] [Google Scholar]
  4. George W. J., Polson J. B., O'Toole A. G., Goldberg N. D. Elevation of guanosine 3',5'-cyclic phosphate in rat heart after perfusion with acetylcholine. Proc Natl Acad Sci U S A. 1970 Jun;66(2):398–403. doi: 10.1073/pnas.66.2.398. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gill G. N., Kanstein C. B. Guanosine 3',5'-monophosphate receptor protein: separation from adenosine 3',5'-monophosphate receptor protein. Biochem Biophys Res Commun. 1975 Apr 21;63(4):1113–1122. doi: 10.1016/0006-291x(75)90684-1. [DOI] [PubMed] [Google Scholar]
  6. Gilman A. G. A protein binding assay for adenosine 3':5'-cyclic monophosphate. Proc Natl Acad Sci U S A. 1970 Sep;67(1):305–312. doi: 10.1073/pnas.67.1.305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Goldberg N. D., O'Dea R. F., Haddox M. K. Cyclic GMP. Adv Cyclic Nucleotide Res. 1973;3:155–223. [PubMed] [Google Scholar]
  8. Green C. D., Martin D. W., Jr A direct, stimulating effect of cyclic GMP on purified phosphoribosyl pyrophosphate synthetase and its antagonism by cyclic AMP. Cell. 1974 Aug;2(4):241–245. doi: 10.1016/0092-8674(74)90017-8. [DOI] [PubMed] [Google Scholar]
  9. Haddox M. K., Nicol S. E., Goldberg N. D. pH induced increase in cyclic GMP reactivity with cyclic AMP-dependent protein kinases. Biochem Biophys Res Commun. 1973 Oct 15;54(4):1444–1450. doi: 10.1016/0006-291x(73)91148-0. [DOI] [PubMed] [Google Scholar]
  10. Hofmann F., Sold G. A protein kinase activity from rat cerebellum stimulated by guanosine-3':5'-monophosphate. Biochem Biophys Res Commun. 1972 Nov 15;49(4):1100–1107. doi: 10.1016/0006-291x(72)90326-9. [DOI] [PubMed] [Google Scholar]
  11. Illiano G., Tell G. P., Siegel M. E., Cuatrecasas P. Guanosine 3':5'-cyclic monophosphate and the action of insulin and acetylcholine. Proc Natl Acad Sci U S A. 1973 Aug;70(8):2443–2447. doi: 10.1073/pnas.70.8.2443. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kuo J. F. Guanosine 3':5'-monophosphate-dependent protein kinases in mammalian tissues. Proc Natl Acad Sci U S A. 1974 Oct;71(10):4037–4041. doi: 10.1073/pnas.71.10.4037. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  14. MARTIN R. G., AMES B. N. A method for determining the sedimentation behavior of enzymes: application to protein mixtures. J Biol Chem. 1961 May;236:1372–1379. [PubMed] [Google Scholar]
  15. Miyamoto E., Kuo J. F., Greengard P. Cyclic nucleotide-dependent protein kinases. 3. Purification and properties of adenosine 3',5'-monophosphate-dependent protein kinase from bovine brain. J Biol Chem. 1969 Dec 10;244(23):6395–6402. [PubMed] [Google Scholar]
  16. Nakazawa K., Sano M. Partial purification and properties of guanosine 3':5'-monophosphate-dependent protein kinase from pig lung. J Biol Chem. 1975 Sep 25;250(18):7415–7419. [PubMed] [Google Scholar]
  17. Schultz G., Hardman J. G., Schultz K., Baird C. E., Sutherland E. W. The importance of calcium ions for the regulation of guanosine 3':5'-cyclic monophosphage levels. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3889–3893. doi: 10.1073/pnas.70.12.3889. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Siegel L. M., Monty K. J. Determination of molecular weights and frictional ratios of proteins in impure systems by use of gel filtration and density gradient centrifugation. Application to crude preparations of sulfite and hydroxylamine reductases. Biochim Biophys Acta. 1966 Feb 7;112(2):346–362. doi: 10.1016/0926-6585(66)90333-5. [DOI] [PubMed] [Google Scholar]
  19. Sold G., Hofmann F. Evidence for a guanosine-3':5'-monophosphate-binding protein from rat cerebellum. Eur J Biochem. 1974 May 2;44(1):143–149. doi: 10.1111/j.1432-1033.1974.tb03467.x. [DOI] [PubMed] [Google Scholar]
  20. Takai Y., Nishiyama K., Yamamura H., Nishizuka Y. Guanosine 3':5'-monophosphate-dependent protein kinase from bovine cerebellum. Purification and characterization. J Biol Chem. 1975 Jun 25;250(12):4690–4695. [PubMed] [Google Scholar]
  21. Van Leemput-Coutrez M., Camus J., Christophe J. Cyclic nucleotide-dependent protein kinases of the rat pancreas. Biochem Biophys Res Commun. 1973 Sep 5;54(1):182–190. doi: 10.1016/0006-291x(73)90906-6. [DOI] [PubMed] [Google Scholar]

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