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. 1976 Aug;73(8):2569–2573. doi: 10.1073/pnas.73.8.2569

In vitro system for induction of delayed early RNA of bacteriophage T4.

C Thermes, P Daegelen, V De Franciscis, E Brody
PMCID: PMC430689  PMID: 785471

Abstract

Concentrated lysates of Escherichia coli that had been infected with bacteriophage T4 in the presence of chloramphenicol show the same restriction of transcription in vitro as is found in vivo. Restricted lysates can be complemented with lysates from infected cells to induce production of delayed early RNA. Complementation takes place between the RNA polymerase of the restricted lysate and the DNA of the unrestricted lysate. We present evidence that delayed early RNA in these lysates is initiated at quasi-late (middle) promoters, and that such recognition is related to changes in the state of the template DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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