Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1976 Aug;73(8):2669–2673. doi: 10.1073/pnas.73.8.2669

Isolation and characterization of the protein coded by gene A of bacteriophage phiX174 DNA.

J E Ikeda, A Yudelevich, J Hurwitz
PMCID: PMC430709  PMID: 1066678

Abstract

Replication of phiX174 circular replicative form (RFI) DNA by extracts of Escherichia coli infected with bacteriophage phiX174 (amber in gene A) requires the phiX174 gene A product. This requirement has been used as an assay for the isolation of this protein. The gene A product (purified 4000-fold) caused relaxation of superhelical phiX174 RFI and formation of discontinuities in the viral strand of phiX174 RFI uniquely situated in the A region of the genome, and yielded a complex after interacting with phiX174 RFI that is active in replication of phiX RFI.

Full text

PDF
2669

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baas P. D., Jansz H. S. Bacteriophage phiX174 DNA synthesis in a replication-deficient host: determination of the origin of phiX DNA replication. J Mol Biol. 1976 Apr 15;102(3):633–656. doi: 10.1016/0022-2836(76)90339-9. [DOI] [PubMed] [Google Scholar]
  2. Baas P. D., Jansz H. S. PhiX174 replicative form DNA replication, origin and direction. J Mol Biol. 1972 Feb 14;63(3):569–576. doi: 10.1016/0022-2836(72)90448-2. [DOI] [PubMed] [Google Scholar]
  3. Clewell D. B., Helinski D. R. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159–1166. doi: 10.1073/pnas.62.4.1159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Denhardt D. T. The single-stranded DNA phages. CRC Crit Rev Microbiol. 1975 Dec;4(2):161–223. doi: 10.3109/10408417509111575. [DOI] [PubMed] [Google Scholar]
  5. Eisenberg S., Denhardt D. T. Structure of nascent phiX174 replicative form: evidence for discontinuous DNA replication. Proc Natl Acad Sci U S A. 1974 Mar;71(3):984–988. doi: 10.1073/pnas.71.3.984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Francke B., Ray D. S. Cis-limited action of the gene-A product of bacteriophage phiX174 and the essential bacterial site (E. coli-electron microscopy-cis-acting protein-specifically-nicked RF). Proc Natl Acad Sci U S A. 1972 Feb;69(2):475–479. doi: 10.1073/pnas.69.2.475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fukuda A., Sinsheimer R. L. Process of infection with bacteriophage phi X 174 XXXVIII. Replication of phi chi 174 replicative form in vivo. J Virol. 1976 Mar;17(3):776–787. doi: 10.1128/jvi.17.3.776-787.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Godson G. N. Origin and direction phiX174 double- and single-stranded DNA synthesis. J Mol Biol. 1974 Nov 25;90(1):127–141. doi: 10.1016/0022-2836(74)90261-7. [DOI] [PubMed] [Google Scholar]
  9. Heil A., Zillig W. Reconstitution of bacterial DNA-dependent RNA-polymerase from isolated subunits as a tool for the elucidation of the role of the subunits in transcription. FEBS Lett. 1970 Dec;11(3):165–168. doi: 10.1016/0014-5793(70)80519-1. [DOI] [PubMed] [Google Scholar]
  10. Henry T. J., Knippers R. Isolation and function of the gene A initiator of bacteriophage phi-chi 174, a highly specific DNA endonuclease. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1549–1553. doi: 10.1073/pnas.71.4.1549. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Johnson P. H., Sinsheimer R. L. Structure of an intermediate in the replication of bacteriophage phiX174 deoxyribonucleic acid: the initiation site for DNA replication. J Mol Biol. 1974 Feb 15;83(1):47–61. doi: 10.1016/0022-2836(74)90423-9. [DOI] [PubMed] [Google Scholar]
  12. Lee A. S., Sinsheimer R. L. A cleavage map of bacteriophage phiX174 genome. Proc Natl Acad Sci U S A. 1974 Jul;71(7):2882–2886. doi: 10.1073/pnas.71.7.2882. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Linney E. A., Hayashi M. N., Hayashi M. Gene A of X174. 1. Isolation and identification of its products. Virology. 1972 Nov;50(2):381–387. doi: 10.1016/0042-6822(72)90389-3. [DOI] [PubMed] [Google Scholar]
  14. Masamune Y., Fleischman R. A., Richardson C. C. Enzymatic removal and replacement of nucleotides at single strand breaks in deoxyribonucleic acid. J Biol Chem. 1971 Apr 25;246(8):2680–2691. [PubMed] [Google Scholar]
  15. Singh S., Ray D. S. A novel single strand endonuclease specific for phiX174 DNA. Biochem Biophys Res Commun. 1975 Dec 15;67(4):1429–1434. doi: 10.1016/0006-291x(75)90186-2. [DOI] [PubMed] [Google Scholar]
  16. Sinsheimer R. L. Bacteriophage phi-X174 and related viruses. Prog Nucleic Acid Res Mol Biol. 1968;8:115–169. [PubMed] [Google Scholar]
  17. Sumida-Yasumoto C., Yudelevich A., Hurwitz J. DNA synthesis in vitro dependent upon phiX174 replicative form I DNA. Proc Natl Acad Sci U S A. 1976 Jun;73(6):1887–1891. doi: 10.1073/pnas.73.6.1887. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wang J. C. Interactions between twisted DNAs and enzymes: the effects of superhelical turns. J Mol Biol. 1974 Aug 25;87(4):797–816. doi: 10.1016/0022-2836(74)90085-0. [DOI] [PubMed] [Google Scholar]
  19. Wickner R. B., Wright M., Wickner S., Hurwitz J. Conversion of phiX174 and fd single-stranded DNA to replicative forms in extracts of Escherichia coli. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3233–3237. doi: 10.1073/pnas.69.11.3233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wickner W., Brutlag D., Schekman R., Kornberg A. RNA synthesis initiates in vitro conversion of M13 DNA to its replicative form. Proc Natl Acad Sci U S A. 1972 Apr;69(4):965–969. doi: 10.1073/pnas.69.4.965. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES